Summary
A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1–2 transformants per μg DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.
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Communicated by W. Gajewski
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Sánchez-Fernández, R., Unkles, S.E., Campbell, E.I. et al. Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase. Molec. Gen. Genet. 225, 231–233 (1991). https://doi.org/10.1007/BF00269853
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DOI: https://doi.org/10.1007/BF00269853