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Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium

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Abstract

Somatic embryos of sunflower (Helianthus annuus) were obtained by placing immature zygotic embryos on a high sucrose (12%) containing medium. The somatic embryos were first observed 6 days after culture and a callus intermediate was not formed. Histological examination revealed the classical stages of embryo development. The somatic embryos proliferated directly from the surface of the zygotic embryos and germinated after placement on a low sucrose medium. Seeds have been obtained from regenerated plants.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

IAA:

Indole-3-acetic acid

BAP:

6-benzylamino purine.

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Authors and Affiliations

  1. Department of Agronomy, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA

    John J. Finer

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  1. John J. Finer
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Communicated by G. C. Phillips

Salaries and research support were provided by State and Federal funds appropriated to OSU-OARDC. Journal Article No. 67–87

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Finer, J.J. Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium. Plant Cell Reports 6, 372–374 (1987). https://doi.org/10.1007/BF00269564

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  • DOI: https://doi.org/10.1007/BF00269564

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