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A transducing bacteriophage λ carrying the structural gene for elongation factor Ts

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Summary

A specialized transducing bacteriophage λdpolCdapD-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and has been verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. Evidence is presented which suggests that the structural genes for ribosomal protein S2 (rpsB) and RNA polymerase σ factor (sit) also lies on λdpolCdapD-9.

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Communicated by H.G. Wittmann

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Friesen, J.D., Parker, J., Watson, R.J. et al. A transducing bacteriophage λ carrying the structural gene for elongation factor Ts. Molec. Gen. Genet. 148, 93–98 (1976). https://doi.org/10.1007/BF00268549

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  • DOI: https://doi.org/10.1007/BF00268549

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