Summary
Irradiation of meiotic yeast cells with moderate doses of ultraviolet irradiation (1,600 erg/mm2) leads to the arrest of premeiotic DNA synthesis, massive (5–40%) DNA degradation, and a 40–50% loss of cell viability. In contrast, such doses of UV irradiation had a minor effect on viability (15–20% loss) of logarithmically growing cells, and no comparable DNA degradation was observed in irradiated synchronized vegetative cells. Meiotic recombination is also affected by UV irradiation. When administered at a stage comparable to meiotic prophase, low doses of irradiation result in a reduction in recombination frequency without significantly affecting cell viability.
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Communicated by W. Gajewski
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Salts, Y., Simchen, G. & Piñon, R. DNA degradation and reduced recombination following UV irradiation during meiosis in yeast (Saccharomyces cerevisiae). Molec. Gen. Genet. 146, 55–59 (1976). https://doi.org/10.1007/BF00267983
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DOI: https://doi.org/10.1007/BF00267983