Summary
Host Factor (HF)1, is a 12000 molecular weight polypeptide that is found in uninfected Escherichia coli and is required as a hexamer along with Qβ replicase for in vitro replication of Qβ phage RNA. It has recently been found to be associated with ribosomes and to bind tightly to poly(A).
We report here the identification and purification of HF from Pseudomonas putida. HF can be detected in crude extracts by both functional activity in the Qβ RNA replication assay and by immunodiffusion with antibody made against E. coli HF. HF from E. coli and P. putida chromatograph similarly on DEAE-cellulose and phosphocellulose. They have similar but not identical molecular weights as judged by SDS-polyacrylamide gel electrophoresis. Like E. coli HF, P. putida HF was found to be associated with ribosomes and to bind tightly to poly (A). Furthermore, the pure protein from P. putida has full functional activity in the in vitro Qβ RNA replication assay.
The findings that HF has been conserved during evolution, is associated with ribosomes, and binds poly(A), suggest that HF may be an important translational element in uninfected cells and that its role involves an interaction with RNA.
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Communicated by W. Gilbert
Research supported by National Institutes of Health Grant GM 21024.
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DuBow, M.S., Blumenthal, T. Host factor for coliphage Qβ RNA replication is present in Pseudomonas putida . Molec. Gen. Genet. 141, 113–119 (1975). https://doi.org/10.1007/BF00267678
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DOI: https://doi.org/10.1007/BF00267678