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Molecular cloning of the gene for the β-lactamase of Bacillus licheniformis and its expression in Escherichia coli

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Summary

The structural gene, pen, for the β-lactamase of B. licheniformis has been cloned into a λ vector and shown to be expressed at a low rate in E. coli. The cloned pen gene appears to be expressed from a promoter within the fragment of B. licheniformis DNA, since its rate of expression is not affected by the presence of the phage repressor, the absence of the phage's positive-control functions, or the position or orientation of the gene within the phage genome.

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Authors and Affiliations

  1. Department of Biochemistry, University of Leicester, University Road, LE1 7RH, Leicester, England

    William J. Brammar & Anne McMorris

  2. Department of Molecular Biology, University of Edinburgh, Mayfield Road, EH9 3JR, Edinburgh, Scotland

    William J. Brammar & Shirley Muir

Authors
  1. William J. Brammar
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  2. Shirley Muir
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  3. Anne McMorris
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Communicated by B.A. Bridges

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Brammar, W.J., Muir, S. & McMorris, A. Molecular cloning of the gene for the β-lactamase of Bacillus licheniformis and its expression in Escherichia coli . Molec. Gen. Genet. 178, 217–224 (1980). https://doi.org/10.1007/BF00267232

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  • DOI: https://doi.org/10.1007/BF00267232

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