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Cloning of the immunity repressor determinant of bacteriophage P2 in the pBR322 plasmid

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Summary

Through in vitro recombination of DNA restriction fragments, we have constructed a plasmid, which expresses in vivo the immunity repressor gene (C) of bacteriophage P2. A bacterial strain carrying such a plasmid showed a high level of P2 specific immunity. It was lysogenized normally by an infecting P2. but the frequency of spontaneous phage production was reduced about 104 fold as compared to a normal P2 lysogen. Satellite phage P4, known to derepress P2 lysogens, was unable to derepress the plasmid-carrying lysogenic strain so to allow growth of coinfecting P2. Phage P4 multiplied on the plasmid-carrying. P2-lysogenic strain, but due to a prolonged latent period failed to form plaques on this strain.

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Authors and Affiliations

  1. Microbial Genetics Laboratory, Karolinska Institutet, S-104 01, Stockholm, Sweden

    Anna Westöö & Elisabeth Ljungquist

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  1. Anna Westöö
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  2. Elisabeth Ljungquist
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Communicated by W. Arber

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Westöö, A., Ljungquist, E. Cloning of the immunity repressor determinant of bacteriophage P2 in the pBR322 plasmid. Molec. Gen. Genet. 178, 101–109 (1980). https://doi.org/10.1007/BF00267218

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  • DOI: https://doi.org/10.1007/BF00267218

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