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Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells

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Summary

The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor (exogenous) DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6·108 dalton of donor DNA per cell (if to assume that all the cells are competent). About 75% of this DNA was found to be attached to the cytoplasmic membrane. Employing Poisson approximation for distribution of the donor-marker DNA molecules on recipient cells it was found that the efficiency of a marker recombination and expression is about 2·10-5

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Authors and Affiliations

  1. Gamaleya Institute for Epidemiology and Microbiology, Moscow, USSR

    A. G. Sabelnikov, A. V. Avdeeva & B. N. Ilyashenko

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  1. A. G. Sabelnikov
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  2. A. V. Avdeeva
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  3. B. N. Ilyashenko
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Communicated by H. Böhme

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Sabelnikov, A.G., Avdeeva, A.V. & Ilyashenko, B.N. Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells. Molec. Gen. Genet. 138, 351–358 (1975). https://doi.org/10.1007/BF00264805

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  • DOI: https://doi.org/10.1007/BF00264805

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