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Modified adipokinetic peptides containing two tryptophan residues and their activities in vitro and in vivo in Locusta

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Abstract

Locusta migratoria has three adipokinetic hormones, adipokinetic hormone-I, II and III. Adipokinetic hormone-III (<QLNFTPWWa) is surprisingly potent (EC50=1.33·10-10 mol·l-1) compared with other adipokinetic hormones (EC50≥5.33·10-10 mol·l-1) at inhibiting acetate uptake into locust fat body in vitro, especially so when it is only moderately potent in mobilizing lipid in vivo. The Trp7 in adipokinetic hormones-III, alongside the Trp8 characteristic of adipokinetic hormones, is not seen in any other adipokinetic hormones. To test whether this is responsible for the high potency of adipokinetic hormone-III in the assay in vitro, novel peptides were synthesised to include or remove this structural motif. Thus, <QLNFTPWWGTa (Trp7-Locusta-adipokinetic hormone-I or [Gly8a-Thr8b]-Locustra-adipokinetic hormone-III); <QLNFTPNWa (des[Gly9-Thr10]-Locusta-adipokinetic hormone-I or Asn7-Locusta-adipokinetic hormone-III); <QLNFSAWWa (Trp7-Locusta-adipokinetic hormone-II) and <QVNFSTWWa (Trp7-Acheta-adipokinetic hormones) were tested both in vitro and in vivo. Except for Trp7-adipokinetic hormone-I in the acetate uptake assay, each of these analogues is less potent then its respective parent, irrespective of the assay. However, the acetate uptake response is highly tolerant of peptides containing Trp7-Trp8, whereas this motif markedly reduces potency in the lipid assay. The different responses exploited in these assays may be exerted through different receptor populations.

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Abbreviations

AKH :

adipokinetic hormones

BSA :

bovine serum albumin

cAMP :

cyclic adenosine monophosphate

EC 50 :

effective concentration giving 50% of effect

FA :

fatty acid(s)

HPLC :

high performance liquid chromatography

RPCH :

red pigment-concentrating hormone

References

  • Baumann E, Penzlin H (1987) Inactivation of neurohormone D by Malpighian tubules in an insect Periplaneta americana. J Comp Physiol B 157: 511–517

    Google Scholar 

  • Baumann E, Gäde G, Penzlin H (1990) Structure-function studies on neurohormone D: activity of naturally-occurring hormone analogues. J Comp Physiol B 160: 423–429

    Google Scholar 

  • Carlisle JA, Loughton BG (1979) Adipokinetic hormone inhibits protein synthesis in Locusta. Nature 282: 420–421

    Google Scholar 

  • Cusinato O, Wheeler CH, Goldsworthy GJ (1991) The identity and physiological actions of an adipokinetic hormone in Acheta domesticus. J Insect Physiol 37: 461–469

    Google Scholar 

  • Gäde G (1992) Structure-activity relationship for the carbohydrate-mobilizing action of further bioanalogues of the adipokinetic hormone/red pigment hormone family of peptides. J Insect Physiol 38: 259–266

    Google Scholar 

  • Gäde G (1993) Structure-activity relationships for the lipid-mobilizing action of further bioanalogues of the adipokinetic hormone/red pigment-concentrating hormone family of peptides. J Insect Physiol 39: 375–383

    Google Scholar 

  • Gäde G, Goldsworthy GJ, Schaffer MH, Cook JC, Rinehart KL (1986) Sequence analyses of adipokinetic hormones II from corpora cardiaca of Schistocerca nitans, Schistocerca gregaria and Locusta migratoria by fast atom bombardment mass spectrometry. Biochem Biophys Res Comm 134: 723–730

    Google Scholar 

  • Gäde G, Reynolds SE, Beeching JR (1994) Molecular evolution of peptides of the AKH-RPCH family. In: Davy et al (eds) Perspectives in endocrinology. Proc XII Int Congr Comp Endocrinol, Toronto, Ontario, 1993. National Research Council of Canada, Ottawa, pp 119–128

    Google Scholar 

  • Gaus G, Kleinholtz LH, Kegel G, Keller R (1990) Isolation and characterization of red pigment-concentrating hormone (RPCH) from six crustacean species. J Comp Physiol B 160: 373–379

    Google Scholar 

  • Gokuldas M, Hunt PA, Candy DJ (1988) The inhibition of lipid synthesis in vitro in the locust Schistocerca gregaria by factors from the corpora cardiaca. Physiol Entomol 13: 43–48

    Google Scholar 

  • Goldsworthy GJ (1983) The endocrine control of flight metabolism in locusts. Adv Insect Physiol 17: 149–204

    Google Scholar 

  • Goldsworthy GJ (1994) The adipokinetic hormones of insects: are they the insect glucagons? In: Davy et al (eds) Perspectives in endocrinology. Proc XII Int Congr Comp Endocrinol, Toronto, Ontario, 1993. National Research Council of Canada, Ottawa, pp 486–492

    Google Scholar 

  • Goldsworthy G, Lee MJ, Luswata R (1995) Adipokinetic hormones: interassay variations in potencies as clues to hormone-receptor interactions in the locust. In: Konopinska D (ed) Insects. Chemical, physiological and environmental aspects 1994. University of Wroclaw, Poland, pp 17–27

    Google Scholar 

  • Goldsworthy GJ, Mallison K, Wheeler CH (1986a) The relative potencies of two known locust adipokinetic hormones. J Insect Physiol 32: 95–101

    Google Scholar 

  • Goldsworthy GJ, Mallison K, Wheeler CH, Gäde G (1986b) Relative adipokinetic activities of members of the adipokinetic hormone/red pigment concentrating hormone family. J Insect Physiol 32: 433–438

    Google Scholar 

  • Hayes TK, Keeley LL (1990) Structure-activity relationships on hyperglycaemia by representatives of the adipokinetic/hyperglycemic hormone family in Blaberus discoidalis cockroaches. J Comp Physiol B 160: 187–194

    Google Scholar 

  • Isaac RE (1988) Neuropeptide-degrading endopeptidase activity of locust (Schistocerca gregaria) synaptic membranes. Biochem J 225: 843–847

    Google Scholar 

  • Kodrík D, Goldsworthy GJ (1995) Inhibition of RNA synthesis by adipokinetic hormones and brain factor(s) in adult fat body of Locusta migratoria. J Insect Physiol 41: 127–133

    Google Scholar 

  • Lee MJ, Goldsworthy GJ (1995a) Acetate uptake assay; the basis of a rapid method for determining potencies of adipokinetic peptides for structure-activity studies. J Insect Physiol 41: 163–170

    Google Scholar 

  • Lee MJ, Goldsworthy GJ (1995b) The preparation and use of dispersed cells from fat body of Locusta migratoria in a filtration plate assay for adipokinetic peptides. Anal Biochem 228: 155–161

    Google Scholar 

  • Mwangi RW, Goldsworthy GJ (1977) Age-related changes on the response to adipokinetic hormone in Locusta. Physiol Entomol 2: 37–42

    Google Scholar 

  • Oudejans RCH, Kooiman FP, Heerma W, Versluis C, Slotboom AJ, Beenakkers AMT (1991) Isolation and structure elucidation of a novel adipokinetic hormone (Lom-AKH-III) from the glandular lobes of the corpus cardiacum of the migratory locust Locusta migratoria. Eur J Biochem 195: 351–359

    Google Scholar 

  • Oudejans RCHM, Dijkhuizen RM, Kooiman FP, Beenakkers AMTh (1992) Dose-response relationships of adipokinetic hormones (Lom-AKH-I II and III) from the migratory locust Locusta migratoria. Proc Exp Appl Entomol 3: 165–166

    Google Scholar 

  • Raina AK, Gäde G (1988) Insect peptide nomenclature. Insect Biochem 8: 785–787

    Google Scholar 

  • Rayne RC, O'Shea M (1992) Inactivation of neuropeptide hormones (AKH I and AKH II) studied in vivo and in vitro. Insect Biochem Mol Biol 22: 25–34

    Google Scholar 

  • Siegert KJ, Mordue W (1992) Degradation of adipokinetic hormones. Insect Biochem Molec Biol 22: 657–663

    Google Scholar 

  • Siegert KJ, Morgan PJ, Mordue W (1985) Primary structures of locust adipokinetic hormone II. Biol Chem Hoppe-Seyler 366: 713–727

    Google Scholar 

  • Stone JV, Mordue W, Batley KE, Morris HR (1976) Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilization during flight. Nature 263: 207–211

    Google Scholar 

  • Stone JV, Mordue W, Broomfield CE, Hardy PM (1978) Structure-activity relationships for the lipid mobilizing action of adipokinetic hormone. Synthesis and activity of a series of hormone analogues. Eur J Biochem 89: 195–202

    Google Scholar 

  • Van Marrewijk WJA, Van Den Broek AThM, Beenakkers AMTh (1980) Regulation of gluconeogenesis in the fat body during flight. Insect Biochem 10: 675–679

    Google Scholar 

  • Van Marrewijk WJA, Van Den Broek AThM, Beenakkers AMTh (1986) Hormonal control of fat body glycogen mobilization for locust flight. Gen Comp Endocrinol 64: 136–142

    Google Scholar 

  • Vroemen SF, Van Marrewijk WJA, Van Der Horst DJ (1995a) Stimulation of glycogenolysis by three locust adipokinetic hormones involves Gs and cAMP. Mol Cell Endocrinol 107: 165–171

    Google Scholar 

  • Vroemen SF, Van Marrewijk WJA, Schepers CCJ, Van Der Horst DJ (1995b) Signal transduction of adipokinetic hormones involves Ca2+ fluxes and depends on extracellular Ca2+ to potentiate cAMP-induced activation of glycogen phosphorylase. Cell Calcium 17: 459–467

    Google Scholar 

  • Ziegler R, Eckart K, Jasensky RD, Law JH (1991) Structure-activity studies on adipokinetic hormones in Manduca sexta. Archs Insect Biochem Physiol 18: 229–237

    Google Scholar 

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Communicated by H. Huddart

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Lee, M.J., Goldsworthy, G.J. Modified adipokinetic peptides containing two tryptophan residues and their activities in vitro and in vivo in Locusta . J Comp Physiol B 166, 61–67 (1996). https://doi.org/10.1007/BF00264640

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