Summary
A WF9 strain of maize with the RD subtype of the S male-sterile cytoplasm (CMS-S) was converted to the inbred M825 nuclear background by recurrent backcrossing. The organization of the mitochondrial genomes of the F1 and succeeding backcross progenies was analyzed and compared with the progenitor RD-WF9 using probes derived from the S1 and S2 mitochondrial episomes, and probes containing the genes for cytochrome c oxidase subunit I (coxI), cytochrome c oxidase subunit II (coxII) and apocytochrome b (cob). Changes in mitochondrial DNA (mtDNA) organization were observed for S1-, S2-, and coxI-homologous sequences that involve loss of homologous restriction enzyme fragments present in the RD-WF9 progenitor. With the coxI probe, the loss of certain fragments was accompanied by the appearance of a fragment not detectable in the progenitor. The changes observed indicate the effect of the nuclear genome on the differential replication of specific mitochondrial subgenomic entities.
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References
Bailey-Serres J, Dixon LK, Liddell AD, Leaver CJ (1986) Nuclearmitochondrial interactions in cytoplasmic male-sterile sorghum. Theor Appl Genet 73:252–260
Borck KS, Walbot V (1982) Comparison of restriction endonuclease digestion patterns of mitochondrial DNA from normal and male sterile cytoplasms of Zea mays L. Genetics 102:109–128
Carlson JE, Gabay-Laughnan S, Laughnan JR (1982) Nucleocytoplasmic interactions in cms-S maize. In: Sheridan WF (ed) Maize for biological research. Plant Mol Biol Assn, Charlottesville, Va. pp 243–245
Dawson AJ, Jones VP, Leaver CJ (1984) The apocytochrome B in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J 3:2107–2113
Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding for cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Escote LJ, Gabay-Laughnan SJ, Laughnan JR (1985) Cytoplasmic reversion to fertility in cms-S maize need not involve loss of linear mitochondrial plasmids. Plasmid 14:264–267
Escote-Carlson LJ, Gabay-Laughnan S, Laughnan JR (1988) Reorganization of mitochondrial genomes of cytoplasmic revertants in cms-S inbred line WF9 in maize. Theor Appl Genet 75:659–667
Fauron C, Havlik M, Lonsdale D, Nichols L (1989) Mitochondrial genome organization of the maize cytoplasmic male sterile type T. Mol Gen Genet 216:395–401
Houchins JP, Ginsburg H, Rohrbaugh M, Dale RMK, Schardl CL, Hodge TP, Lonsdale DM (1986) DNA sequence analysis of a 5.27 kb direct repeat occurring adjacent to the regions of S-episome homology in maize mitochondria. EMBO J 5:2781–2788
Isaac PG, Jones VP, Leaver CJ (1985) The maize cytochrome c oxidase I gene: Sequence, expression and rearrangement in cytoplasmic male sterile plants. EMBO J 4:1617–1623
Ishige T, Storey KK, Gengenbach BG (1985) Cytoplasmic fertile revertants possessing S1 and S2 DNAs in S male sterile maize. Jpn J Breed 35:285–291
Kemble RJ, Mans RJ (1983) Examination of the mitochondrial genome of revertant progeny from cms-S maize with cloned S1 and S2 hybridization probes. J Mol Appl Genet 2:161–171
Laughnan JR, Gabay-Laughnan SJ, Carlson JE (1981) Characteristics of cms-S reversion to male fertility in maize. Stadler Genet Symp 13:93–114
Leaver CJ, Isaac PG, Bailey-Serres J, Small ID, Hanson DK, Fox TD (1985) Recombination events associated with the cytochrome c oxidase subunit I gene in fertile and cytoplasmic male sterile maize and sorghum. In: Quagliariello E, Slater EC, Palmieri F, Saccone C, Kroon AM (eds) Achievements and perspectives of mitochondrial research, vol. 2. Biogenesis. Elsevier, Amsterdam, pp 111–122
Leaver CJ, Isaac PG, Small ID, Bailey-Serres J, Liddell AD, Hawkesford MJ (1988) Mitochondrial genome diversity and cytoplasmic male sterility in higher plants. Philos Trans R Soc Lond [Biol] 319:165–176
Levings III CS, Kim BD, Pring DR, Conde MF, Mans RJ, Laughnan JR, Gabay-Laughnan SJ (1980) Cytoplasmic reversion of cmsS in maize: Association with a transpositional event. Science 209:1021–1023
Lonsdale DM (1984) A review of the structure and organization of the mitochondrial genome of higher plants. Plant Mol Biol 3:201–206
Lonsdale DM, Hodge TP, Fauron CMR (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12:9249–9261
Lonsdale DM, Brears T, Hodge TP, Melville SE, Rottman WH (1988) The plant mitochondrial genome: homologous recombination as a mechanism for generating heterogeneity. Philos Trans R Soc Lond [Biol] 319:149–163
Palmer JD (1988) Intraspecific variation and multicircularity in Brassica mitochondrial DNAs. Genetics 118:341–351
Palmer JD, Herbon LA (1986) Tricircular mitochondrial genomes of Brassica and Raphanus: reversal of repeat configurations by inversion. Nucleic Acids Res 14:9755–9764
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307:437–440
Pring DR, Lonsdale DM (1985) Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97:1–46
Quetier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) Molecular form and function of the wheat mitochondrial genome. In: Van Vloten-Doting L, Groot G, Hall T (eds) Molecular form and function of the plant genome. Plenum Press, New York, pp 413–420
Schardl CL, Lonsdale DM, Pring DR, Rose KR (1984) Linearization of maize mitochondrial chromosomes by recombination with linear episomes. Nature 310:292–296
Schardl CL, Pring DR, Lonsdale DM (1985) Mitochondrial DNA rearrangements associated with fertile revertants of S-type male sterile maize. Cell 43:361–368
Siculella L, Palmer JD (1988) Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMSassociated alterations in structure and transcription of the atpA gene. Nucleic Acids Res 16:3787–3799
Small ID, Isaac PG, Leaver CJ (1987) Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J 6:865–869
Small ID, Earle ED, Escote-Carlson LJ, Gabay-Laughnan S, Laughnan JR, Leaver CJ (1988) A comparison of cytoplasmic revertants to fertility from different cms-S maize sources. Theor Appl Genet 76:609–618
Stern DB, Palmer JD (1986) Tripartite mitochondrial genome of spinach: Physical structure, mitochondrial gene mapping, and locations of transposed chloroplast DNA sequences. Nucleic Acids Res 14:5651–5666
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Escote-Carlson, L.J., Gabay-Laughnan, S. & Laughnan, J.R. Nuclear genotype affects mitochondrial genome organization of CMS-S maize. Molec. Gen. Genet. 223, 457–464 (1990). https://doi.org/10.1007/BF00264454
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DOI: https://doi.org/10.1007/BF00264454