Skip to main content
SpringerLink
Log in

Binding of 99mTc-gluconate to heart mitochondria

  • Published:
European Journal of Nuclear Medicine Aims and scope Submit manuscript

Abstract

99mTc-gluconate has previously been shown to bind to isolated rat heart mitochondria. In the presence of potassium cyanide this binding is enhanced several fold, an effect which has been shown to be correlated to the efficiency of the in vivo binding of the isotope complex in ischemic dog hearts. The present investigation shows that the potassium cyanide-induced binding was mainly localized to the mitochondrial inner membrane; in the presence of cyanide other organelles, e.g., nuclei and liver microsomes also showed some binding. Boiling, lipid extraction, or addition of N-ethylmaleimide caused a variable inhibition of the binding of 99mTc-gluconate to rat heart mitochondria, which also was markedly influenced by temperature, pH, time, and concentrations of protein and isotope complex, but not by conditions affecting energy levels or calcium transport. Fractionation of submitochondrial particles exposed to 99mTc-gluconate in the presence of potassium cyanide indicated that the 99mTc-gluconate-binding component cofractionated with cytochrome oxidase. It is postulated that a protein component localized in the mitochondrial inner membrane, possibly cytochrome oxidase, is responsible for the binding of 99mTc-gluconate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ahlberg N-E, Bois-Svensson I, calissendorff B, Lewander R, Lundqvist H, Rajs I (1978) Uptake of Tc-99m-gluconate and early histologic findings in induced myocardial infarcts in dogs. Acta Radiol [Diagn] (Stockh) 18:218–224

    Google Scholar 

  2. Ahlberg N-E, Cronestrand R, Galaris D, Grandinsson M, Rydström J (1982) Subcellular binding of Tc-99m-gluconate in infarcted myocardium in dogs. Acta Radiol [Oncol] 20:357–360

    Google Scholar 

  3. Berezny R, Crane FL (1971) Cytochromes in bovine liver nuclear membranes. Biochem Biophys Res Commun 43:1017–1023

    Google Scholar 

  4. Blobel G, van Potter R (1966) Nuclei from rat liver: Isolation method that combines purity with high yield. Science 154:1662–1665

    Google Scholar 

  5. Boyd RE, Robinson J, Hunt FC, Sorby PJ, Murray IPC, McKay WJ (1973) Tc-99m-gluconate complexes for renal scintigraphy. Br J Radiol 46:604–612

    Google Scholar 

  6. Buja LM, Parney RW, Bees JM, Stokely EM, Harris RA, Bonte FJ, Willerson JT (1975) Morphologic correlates of technitum-99 stannous pyrophosphate imaging of acute myocardial infarcts in dogs. Circulation 52:596–607

    Google Scholar 

  7. Buja LM, Tofe AJ, Kulkarni PV, Mukhejee A, Parkey RW, Francis MD, Bonte FJ, Willerson JT (1977) Sites and mechanisms of localization of technetium-99m phosphorus radiopharmaceuticals in acute myocardial infarcts and other tissues. J Clin Invest 60:724–740

    Google Scholar 

  8. Bygrave FL (1977) Mitochondrial calcium transport. In: Current Topics Bioenerg, vol. 6. (ed.) Sanadi, R. Academic Press, London pp. 259–318

    Google Scholar 

  9. D'Agostino AN (1964) An electron microscope study of cardiac necrosis produced by 9-a-fluorocortisol and sodium phosphate. Am J Pathol 45:633–644

    Google Scholar 

  10. D'Agostino AN, Chiga M (1970) Mitochondrial mineralization in human myocardium. Am J Clin Pathol 58:820–824

    Google Scholar 

  11. Deutsch E, Bushong W, Glaran KA, Elder RC, Sodd VJ, Scholz KL, Forman DL, Lukes SJ (1981) Heart imaging with cationic complexes of technetium. Science 214:85–86

    Google Scholar 

  12. Ernster L, Siekewitz P, Palade GE (1962) Enzyme-structure relationships in the endoplasmatic reticulum of rat liver. A morphological and biochemical study. J Cell Biol 15:541–562

    Google Scholar 

  13. Fleischer S, Fleischer B (1967) Removal and binding of polar lipids in mitochondria and other membrane systems. Methods Enzymol 10:406–433

    Google Scholar 

  14. Friedman E, Marrian DH, Simon-Reuss I (1949) Antimitotic action of maleimide and related substances. Br J Pharmacol 4:105–110

    Google Scholar 

  15. Hogeboom GH (1955) Fractionation of cell components of animal tissues. Methods Enzymol 1:16–19

    Google Scholar 

  16. Höchli L, Hackenbrock CR (1978) Cytochrome c oxidase from rat liver mitochondria: Purification and characterization. Biochemistry 17:3712–3719

    Google Scholar 

  17. Klein MS, Coleman RE, Ahmed SA, Weiss ES, Roberts R, Sobel BE (1975) Tc-99m (Sn) pyrophosphate scintigraphy: Sensitivity specificity and mechanisms. Circulation 51:(Suppl II) 52

    Google Scholar 

  18. Klein MS, Roberts R, Coleman RE (1978) Radionuclides in the assessment of myocardial infarction. Am Heart J 95:659–667

    Google Scholar 

  19. Lee CP, Ernster L (1967) Energy-coupling in nonphosphorylating submitochondrial particles. Methods Enzymol 10:543–548

    Google Scholar 

  20. Löw H, Vallin I (1963) Succinate-linked diphosphopyridine nucleotide reduction in submitochondrial particles. Biochim Biophys Acta 69:361–366

    Google Scholar 

  21. Means GE, Feeney RE (1971) Chemical modification of proteins. Holden-Day, Inc., San Francisco London

    Google Scholar 

  22. Moore CL (1971) Specific inhibition of mitochondrial Ca2+ transport by ruthenium red. Biochem Biophys Res Commun 42:298–305

    Google Scholar 

  23. Moravec I, Renault G, Hatt PY (1978) Alterations of mitochondrial function as detected in left ventricular myocardium of rats with acute aortic constriction. Basic Res Cardiol 73:535–550

    Google Scholar 

  24. Orii Y, Yoshikawa S Inhibitors acting on cytochrome oxidase. In: Oxidases and related redox systems. King T, Mason HS, Morrison M (eds), University Park Press, London, pp 649–663

  25. Rajs I, Ahlberg N-E, Orrenius S, Söderlund U (1979) Accumulation of Tc-99m-gluconate in isolated rat cardial myocytes and hepatocytes during anoxia. Acta Radiol Oncol 18:218–224

    Google Scholar 

  26. Rajs I, Sundberg M, Härm T, Grandinsson M, Söderlund U (1980) Effects of anoxia and reoxygenation on isolated rat cardiac myocytes. J Mol Cell Cardiol 12:1227–1238

    Google Scholar 

  27. Raison J (1973) The influence of temperature-induced phase changes on the kinetics of respiratory and other membraneassociated enzyme systems. J Bioenerg Biomembr 4:285–309

    Google Scholar 

  28. Shen AC, Jennings RB (1972) Kinetics of calcium accumulation in acute myocardial ischemic injury. Am J Pathol 67:441–452

    Google Scholar 

  29. Strauss HW, Pitt B (1978) Evaluation of cardiac function and structure with radioactive tracer techniques. Circulation 57:645–654

    Google Scholar 

  30. Sundrehagen E (1970) Polymer formation and hydrolysation of Tc-99(IV). Int J Appl Radiat Isot 30:739–743

    Google Scholar 

  31. Tyler DD, Gonze J (1967) The preparation of heart mitochondrion from laboratory animals. Methods Enzymol 10:75–77

    Google Scholar 

  32. Vercesi A, Reynafarje B, Lehninger AL (1978) Stoichiometry of H+ ejection and Ca2+ uptake coupled to electron transport in rat heart mitochondria. J Biol Chem 253:6370–6385

    Google Scholar 

  33. Wharton DC, Tzagoloff A (1967) Cytochrome oxidase from beef heart mitochondria. Methods Enzymol 10:245–250

    Google Scholar 

  34. Yonetani T (1961) Studies on cytochrome oxidase. J Biol Chem 236:1680–1688

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91, Stockholm, Sweden

    D. Galaris &  J. Rydström

  2. The Department of Diagnostic Radiology, Huddinge Hospital, S-141 86, Huddinge, Sweden

    M. Grandinson, U. Söderlund & N. E. Ahlberg

Authors
  1. D. Galaris
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Grandinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. U. Söderlund
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. E. Ahlberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Rydström
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Galaris, D., Grandinson, M., Söderlund, U. et al. Binding of 99mTc-gluconate to heart mitochondria. Eur J Nucl Med 8, 4–9 (1983). https://doi.org/10.1007/BF00263505

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00263505

Keywords

Search

Navigation