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Investigation of the coupling of water soluble poly(acrylic acid) and poly(methacrylic acid) to amines and to β-galactosidase

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Summary

Acrylic acid and methacrylic acid were polymerized by γ — irradiation to give homogeneous solution of homopolymer. Neither homopolymeric species coupled to benzylamine or phenylpropylamine with water soluble carbodi-imides or Woodward's K Reagent but both homopolymers coupled to β — galactosidase using a water soluble carbodi-imide. In each instance, the activity of the enzyme was unaffected. Evidence for the formation of covalent links between the enzyme and the copolymer was obtained from electrophoresis and by analyzing the products obtained by precipitation of the enzyme-copolymer complexes. Both homopolymer species markedly effected the activity of acid phosphatase without the condensing agent being present.

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Authors and Affiliations

  1. School of Health and Applied Science, Leeds Polytechnic, Calverley Street, LS1 3HE, Leeds, UK

    C. G. Beddows

  2. Department of Colour Chemistry, University of Leeds, LS2 9JT, Leeds, UK

    P. S. Barker & J. T. Guthrie

Authors
  1. C. G. Beddows
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  2. P. S. Barker
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  3. J. T. Guthrie
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Beddows, C.G., Barker, P.S. & Guthrie, J.T. Investigation of the coupling of water soluble poly(acrylic acid) and poly(methacrylic acid) to amines and to β-galactosidase. Polymer Bulletin 4, 149–156 (1981). https://doi.org/10.1007/BF00263055

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  • DOI: https://doi.org/10.1007/BF00263055

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