Summary
Isotocinergic (IT) neurons show physiological and morphological characteristics that are similar to those of other preoptic neuroendocrine cells in the goldfish. Preoptic IT cells show resting membrane potentials of 20–55 mV, action potentials of up to 100mV, and physiological evidence of axonal branching. Dye-marked IT cells measure 14–56 μm, their dendrites projecting to the ependyma and into the hypothalamic neuropil, their multiple beaded axons projecting to the pituitary. Indirect immunofluorescence identifies these dyemarked cells as IT. By combining electrophysiological, dye-marking and immunocytochemical techniques we can now, for the first time, study single, antidromically-identified peptidergic neurons of a specific type in vertebrate and invertebrate species.
Similar content being viewed by others
References
Brown, K.T., Flaming, D.G.: Instrumentation and technique for beveling fine micropipette electrodes. Brain Res. 86, 172–180 (1975)
Coons, A.H.: Fluorescent antibody methods. In: General Cytochemical Methods, pp. 399–422, (J.F. Danielli, ed.) New York: Academic Press, 1958
Eccles, J.C.: The Physiology of Synapses. New York: Academic Press, 1964
Hayward, J.N.: Physiological and morphological identification of hypothalamic magnocellular neuroendocrine cells in goldfish preoptic nucleus. J. Physiol. (Lond.) 239, 103–124 (1974)
Hayward, J.N.: Functional and morphological aspects of hypothalamic neurons. Physiol. Rev. 57, 574–658 (1977)
Kandel, E.R.: Electrical properties of hypothalamic neuroendocrine cells. J. Gen. Physiol. 47, 691–717 (1964)
Koizumi, K., Yamashita, H.: Studies of antidromically identified neurosecretory cells of the hypothalamus by intracellular and extracellular recordings. J. Physiol. (Lond.) 221, 683–705 (1972)
Reaves, T.A., Jr., Hayward, J.N.: Chemical identification of physiologically-defined, dye-injected neuroendocrine cells in preoptic nucleus of goldfish hypothalamus. Endocrine Soc. Abst. 61, 160 (1979a)
Reaves, T.A., Jr., Hayward, J.N.: Immunocytochemical identification of enkephalinergic neurons in the hypothalamic magnocellular preoptic nucleus of the goldfish, Carassius auratus. Cell Tissue Res. 200, 147–151 (1979b)
Sawyer, W.H., Pang, P.K.T.: Evolution of neurohypophysial hormones and their functions. In: Neurohypophysis, pp. 1–8 (A.M. Moses and L. Share, eds.) Basel: S. Karger, 1977
Stewart, W.W.: Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell 14, 741–759 (1978)
Vandesande, F., Dierickx, K., DeMay, J.: The origin of vasopressinergic and oxytocinergic fibers of the external region of the median eminence of the rat hypophysis. Cell Tiss. Res. 180, 443–452 (1977)
Young, J.Z.: The preparation of isotonic solutions for use in experiments with fish. Pubbl. Staz. Zool. Napoli 12, 425–431 (1933)
Author information
Authors and Affiliations
Additional information
Supported by Grants from the USPHS (NS-13411 and NS-05696)
The authors wish to thank Ms. S. Curtis for editorial assistance, Ms. D. Cronce for skillful technical assistance, Dr. W.W. Stewart for helpful suggestions and for his generous gift of Lucifer Yellow-CH, Dr. M. Manning for his generous gift of high quality peptides, and Dr. R.R. Dries and J.D. Fernstrom for kindly supplying antisera
Rights and permissions
About this article
Cite this article
Reaves, T.A., Hayward, J.N. Isotocinergic neurons in the goldfish hypothalamus: Physiological and morphological studies on chemically identified cells. Cell Tissue Res. 202, 17–23 (1979). https://doi.org/10.1007/BF00239217
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00239217