Summary
The relationship of oxygen consumption with the activity of the synaptic Na-K-pump was investigated in rat cortical synaptosomes. Changes in oxygen consumption were monitored during the restitution of physiological ionic equilibrium in incubated synaptosomes and under the effects of ouabain and protoveratrine. The ouabain-sensitive component of synaptosomal respiration was measured and correlated with earlier data on synaptosomal K+-uptake. This ouabain-sensitive component was shown to reflect the activity of the Na-K-pump. Our results thus provide a means for the continuous monitoring of ion-pump activity in intact synaptosomes.
Similar content being viewed by others
References
Blaustein MP, Goldring JM (1975) Membrane potentials in pinched-off presynaptic nerve terminals monitored with fluorescent probe: evidence that synaptosomes have potassium diffusion potentials. J Physiol (Lond) 247: 589–615
Bradford HF (1969) Respiration in vitro of synaptosomes from mammalian cerebral cortex. J Neurochem 16: 675–684
Bradford HF (1970) Metabolic responses of synaptosomes to electrical stimulation release of amino acids. Brain Res 19: 239–247
Bradford HF, Bennett CW, Thomas AJ (1973) Depolarizing stimuli and the release of physiologically active amino acids from suspensions of mammalian synaptosomes. J Neurochem 21: 495–505
Bradford HF, Jones DG, Booher J (1975) Biochemical and morphological studies of the short and long term survival of isolated nerve endings. Brain Res 90: 245–249
Bradford HF, Ward HK, Thomas AJ (1978) Glutamine — a major substrate for nerve endings. J Neurochem 30: 1453–1459
Csillag A, Hajós F (1980) Potassium movements in relation to synaptosomal morphology. J Neurochem 24: 495–503
Diamond I, Fishman RA (1973) Development of Na-stimulated oxidation in synaptosomes. J Neurochem 21: 1043–1050
Hajós F (1975) An improved method for the preparation of synaptosomal fractions in high purity. Brain Res 93: 485–489
Kálmán M, Hajós F (1982) Comparative studies fresh and postmortem isolated rat cortical synaptosomes: morphology, K-transport and respiration. J Neurochem 39: 1740–1742
Lowry OH, Rosebrough NJ, Tarr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Pastuszko A, Wilson DF, Erecinska M, Silver IA (1981) Effects of in vitro hypoxia and lowered pH on potassium fluxes and energy metabolism in rat brain synaptosomes. J Neurochem 36: 116–123
Scott ID, Nicholls DG (1980) Energy transduction in intact synaptosomes. Influence of plasma membrane depolarisation on the respiration and membrane potential of internal mitochondria determined in situ. Biochem J 186: 21–33
Thomas RC (1972) Electrogenic sodium pump in nerve and muscle cells. Physiol Rev 52: 563–594
Tozalakian PV, Csillag A (1982) The effect of γ-aminobutyric acid on the potassium movements of rat cortical synaptosomes. Exp Brain Res 46: 24–48
Verity MA (1972) Cation modulation of synaptosomal respiration. J Neurochem 19: 1305–1317
Wedege E, Luqmani Y, Bradford HF (1977) Stimulated incorporation of amino acids into proteins of synaptosomal fractions induced by depolarizing treatments. J Neurochem 29: 527–537
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kálmán, M. The ouabain-sensitive component of synaptosomal respiration. Exp Brain Res 56, 539–542 (1984). https://doi.org/10.1007/BF00237995
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00237995