Summary
A method was developed that allows the analysis of neuropeptides and monoamines in a single tissue section by the application of the unlabeled antibody method for peptide staining to tissue sections freeze-dried for formaldehyde-induced monoamine histofluorescence. The hypothalamic magnocellular system of male albino rats served as a model for this study; neurons were stained with anti-neurophysin sera, which mark the vasopressin- and oxytocin-associated proteins. Neurophysin-containing perikarya appeared to be surrounded by catecholamine-containing varicosities. This phenomenon was seen to varying degrees within the supraoptic and paraventricular nuclei. The juxtaposition of varicosities and peptidergic neurons suggests an afferent fiber-target neuron relationship that might favor a functional interaction between monoamines and neuropeptides.
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Supported by USPHS Grant and postdoctoral NS15816 (JRS) fellowship AG01575 (THM)
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Sladek, J.R., McNeill, T.H. Simultaneous monoamine histofluorescence and neuropeptide immunocytochemistry. Cell Tissue Res. 210, 181–189 (1980). https://doi.org/10.1007/BF00237608
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DOI: https://doi.org/10.1007/BF00237608