Skip to main content
Log in

The role of the conversion of IBA into IAA on root regeneration in apple: introduction of a test system

Plant Cell Reports Aims and scope Submit manuscript

Abstract

A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid (IBA) or indoleacetic acid (IAA) induced maximally an average of 8 or 4.5 roots per slice, respectively. After uptake of IBA, a high degree of conjugation resulted in a recovery of 2.5% as internal free IBA (ca. 2 times the medium concentration). Due to conversion of absorbed IBA into IAA a fraction of 0.4% was recovered as (physiologically active) free IAA. After incubation on medium with IAA, 0.5% of the absorbed hormone was recovered in the free acid form. No conversion of IAA into IBA was observed. Equimolar contents of internal free IAA after incubation on IBA or IAA resulted for IBA in a higher number of roots than for IAA. This means that IBA may also act via internal free IBA or may synergistically modify the action or endogenous synthesis of IAA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BHT:

butyl hydroxy toluene

EtOH:

ethanol

FW:

fresh weight

HAc:

acetic acid

HPLC:

high performance liquid chromatography

IAA:

indoleacetic acid

IAAint :

free (internal) IAA in the tissue derived from IBA or IAA uptake

IBA:

indolebutyric acid

IBAint :

free (internal) IBA in the tissue derived from IBA or IAA uptake

MeOH:

methanol

PAR:

photosynthetic active radiation

SEM:

standard error of the mean

TLC:

thin layer chromatography

UV:

ultra violet

References

  • Alvarez R, Nissen SJ, Sutter EG (1989) J Plant Growth Regul 8: 263–272

    Google Scholar 

  • Andreae WA, Good NE (1957) Plant Physiol 32: 566–572

    Google Scholar 

  • Cohen JD, Bandurski RS (1982) Annu Rev Plant Physiol 33: 403–430

    Article  CAS  Google Scholar 

  • De Klerk GJ, Smulders R, Benschop M (1990) Acta Hortic 280: 29–36

    Google Scholar 

  • Epstein E, Lavee S (1984) Plant Cell Physiol 25: 679–703

    Google Scholar 

  • Guern J (1987) Ann Bot 60: 75–102

    Google Scholar 

  • Hangarter RP, Good NE (1981) Plant Physiol 68: 1424–1427

    CAS  Google Scholar 

  • James DJ, Thurbon IJ (1981) J Hortic Sci 56: 15–20

    Google Scholar 

  • Ludwig-Müller J, Epstein E (1991) Plant Physiol 97: 765–770

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15: 473–497

    CAS  Google Scholar 

  • Nordström AC, Alvarado Jacobs F, Eliasson L (1991) Plant Physiol 96: 856–861

    Google Scholar 

  • Pythoud F, Buchala AJ (1989) Plant Cell Environ 12: 489–494

    Google Scholar 

  • Quoirin M, Lepoivre P, Boxus Ph (1977) In C.R. Rech. 1976–1977 et Rapports de Synthèse Stat Fruit et Maraîch. Gembloux. Belgium: 93–117

  • Smulders MJM, Visser EJW, Van der Krieken WM, Croes AF, Wullems GJ (1990) Plant Physiol 92: 582–586

    Google Scholar 

  • Van der Krieken WM, Breteler H, Visser MHM (1992a) Plant Cell Physiol 33: 709–713

    Google Scholar 

  • Van der Krieken WM, Breteler H, Visser MHM, Jordi W (1992b) Physiol Plant. 85: 589–594

    Google Scholar 

  • Van der Krieken WM, Breteler H, Visser MHM (1992c) Acta Bot Neerl 41:(in the press)

  • Welander M (1983) Physiol Plant. 58: 231–238

    Google Scholar 

  • Wiesman Z, Riov J, Epstein E. (1988) Physiol Plant 74: 556–560

    Google Scholar 

  • Wiesman Z, Riov J, Epstein E. (1989) Plant Physiol 91: 1080–1084

    Google Scholar 

  • Zimmerman RH (1981) Plant Cell Tiss Qrg Cult 3: 301–311

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by W. Barz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Van der Krieken, W.M., Breteler, H., Visser, M.H.M. et al. The role of the conversion of IBA into IAA on root regeneration in apple: introduction of a test system. Plant Cell Reports 12, 203–206 (1993). https://doi.org/10.1007/BF00237054

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00237054

Keywords

Navigation