Summary
Unilateral stimulation of the lateral-line organ (LLO) of the teleost Scardinius erythrophthalmus by current water caused an increase in the afferent fibre activity in the stimulated organ of about 3.5 times as compared with the non-stimulated LLO.
There was an increase of [3H] histidine incorporation as compared to controls following stimulation applied either at the beginning (1 h) or at the end (1.5–6 h) of various incorporation times.
Following a 1 h stimulation period, the different areas of the LLO-system (lateral nerve, medulla oblongata, cerebellum and valvulae cerebelli), as well as the optic tectum, subtectum and spinal cord showed a significant increase of protein labelling; whereas after 12 h post-incorporation times only the lateral nerve showed highly significant differences as compared to controls.
In animals stimulated at the end of a 12 h pre-incorporation period (1.5–6 h) there was a significant increase of protein labelling in all investigated structures of the brain as compared to controls.
Similar content being viewed by others
References
Alnes, E.: Two types of lateral line afferents in the eel (Anguella anguilla). Acta physiol. scand. 87, 535–548 (1973a)
Alnes, E.: Peripheral inhibition of eel lateral line receptors as caused by antidromic sensory invasion. Acta physiol. scand. 88, 35–48 (1973b)
Alnes, E.: Lateral line input to the crista cerebellaris in the eel field potentials and histology. Acta physiol. scand. 88, 49–61 (1973c)
Alnes, E.: Unit activity of ganglionic and medullary second order neurones in the eel lateral line system. Acta physiol. scand. 88, 160–174 (1973d)
Altman, J.: Differences in the utilisation of tritiated leucine by single neurones in normal and exercisel rats: an autoradiographic investigation with microdensitometry. Nature (Lond.) 199, 777–780 (1963)
Aronson, L.R.: The central nervous system of sharks and bony fishes with special reference to sensory and integrative mechanisms. In:Sharks and survival. (P.W. Gilbert Ed.) Boston: D.C. Heath and Company 1963
DeLamater, E., Courtenay, Jr., W.R.: Variations in structure of the lateral-line canal on scales of teleostean fishes. Z. Morphologie der Tiere. 75, 259–266 (1973)
Denny, H.: The lateral-line system of teleosts. J. comp. Neurol. 68, 49–65 (1937)
Dijkgraaf, S.: The functioning and significance of the lateral-line organs. In: Biological Review of the Cambridge Philosophical Society. Vol. 38. (H.M. Fox Ed.). p. 51–105. Cambridge: University Press 1963
Edström, J.E.: Effects of increased motor activity on the dimensions and the staining properties of the neuron soma. J. comp. Neurol. 107, 295–304 (1957)
Flock, A.: Ultrastructure and functioning of the lateral line organs. In: Lateral line detectors. (P.H. Calm Ed.) p. 163–197. Bloomington: Indiana University Press 1967
Hama, K., Katsuki, Y., Hashimoto, T., Yanagisawa, K.: The lateral-line organ of shark as chemoreceptor. Advance in Biophys. 1, 1–51 (1970)
Harris, G.G., Bergeijk, W.A. van: Evidence that the lateral-line organ responds to near-field displacements of sound sources in water. J. acoust. Soc. Am. 34, 1831–1841 (1962)
Hyden, H., Eghjazi, E.: Nuclear RNA changes of nerve cells during a learning experiment in rats. Proc. nat. Acad. Sci. (Wash.) 48, 1366–1373 (1962)
Katsuki, Y., Yoshino, S., Chen, I.: Neural mechanisms of the lateral-line organs of fish. Jap. J. Physiol. 1, 264–268 (1951)
Katsuki, Y., Hashimoto, F., Yanagisawa, K.: Information processing in fish lateral-line sense organs. Science (N. Y.) 160, 439 (1968)
Katsuki, Y., Hashimoto, T., Kendall, I.I.: The chemoreception in the lateral-line organs of teleosts. Jap. J. Physiol. 21, 99–118 (1971)
Kroker, H.: Autoradiographische Untersuchungen über die Protein- und RNS-Synthese im Tectum opticum von Karauschen (Carassius carassius L.) nach Lichtreizung. Z. mikrosk.-anat. Forsch. (Leipzig) 87, 525–543 (1973)
Lim, R., Agranoff, B.W.: Protein metabolism in goldfish brain. J. Neurochem. 16, 431–445 (1969)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Maler, L.: The Posterior Lateral Line Lobe of a Mormyrid Fish — A Golgi Study. J. comp. Neurology 152, 281–298 (1973)
Orrego, F., Lipman, F.: Protein synthesis in brain slices. Effects of electrical stimulation and acidic amino acids. J. Biol. Chem. 242, 665 (1967)
Rahmann, H., Hilbig, R.: Autoradiographische Untersuchungen über Stoffwechselunterschiede in verschiedenen Hirnstrukturen von Teleosteern sowie deren Beinflußbarkeit nach motorischer Stimulation. Z. Zellforsch. 133, 501–518 (1972)
Rahmann, H., Rösner, H., Wilhelm, R.: Changes in protein synthesis in fish brain induced by electrical stimulation. IRCS Medial Sciences 3-10-31, 73–11 (1973)
Rahmann, H., Jansen, B.: Biochemische Untersuchungen des Proteinstoffwechsels im ZNS von Teleosteern. Univ. Hohenheim/Stuttgart 1975
Rahmann, H., Hilbig, R.: Autoradiographische Untersuchungen zum Einfluß von unilateralen Strömungsstimulationen auf den Einbau von 3H-Histidine in die verschiedenen Substrukturen des Seitenlinienorgan-Systems von Teleosteern. Zoolog. Jahrbücher. Abt. Physiol. 71, 518–527 (1975)
Rensch, B., Rahmann, H.: Autoradiographische Untersuchungen über visuelle „Engramm”-Bildung bei Zahnkarpfen. Pflügers Arch. 290, 158–166 (1966)
Rensch, B., Rahmann, H., Skrzipek, H.: Autoradiographische Untersuchungen über visuelle „Engramm”-Bildung bei Fischen. Pflügers Arch. 304, 242–252 (1968)
Roberts, B.L., Russell, I.J.: The Activity of Lateral-Line Efferent Neurones in Stationary and Swimming Dogfish. J. Exp. Biol. 57, 435–448 (1972)
Rose, S.P.R.: Neurochemical correlates of learning and environmental changes. FEBS Letters, 5, 305–312 (1969)
Russell, I.J., Roberts, B.L.: Inhibition of spontaneous Lateral-Line activity by efferent nerve stimulation J. Exp. Biol. 57, 77–82 (1972)
Schacht, J., Agranoff, B.W.: Phospholipid labelling by 32P-orthophosphate, and 3H-myo-inosital in the stimulated goldfish brain in vivo. J. Neurochem. 19, 1417–1421 (1972)
Tiplady, B.: Brain protein metabolism and environmental stimulation, effects of forced exercise. Brain Res. 43, 215–225 (1972)
Watson, W.E.: An autoradiographic study of the incorporation of nucleic acid precursors by neurones and glia during nerve stimulation. J. Physiol. (Lond.) 180, 741–753 (1965)
Wawrzyniak, M.: Chemoarchitektonische Studien am Tectum opticum von Teleosteern unter normalen und experimentellen Bedingungen. Z. Zellforsch. 58, 234–264 (1962)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hilbig, R., Rahmann, H. Changes in the protein metabolism of the CNS of a teleost following stimulation of the lateral-line organ. Exp Brain Res 26, 249–260 (1976). https://doi.org/10.1007/BF00234930
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00234930