Summary
Embryogenic cultures of interior spruce derived from 12 full-sib families were subjected to cryopreservation, with a 97 % success rate for 357 genotypes. Analyses suggested that cryotolerance was not related to family ranking (height increment), embryogenic potential or culture dispersability in suspension, and long-term storage in or above liquid nitrogen did not affect regenerative potential. By contrast, differences in cryotolerance among cell lines appeared to be prevalent in certain families. Analysis with a DNA fingerprinting probe used for clonal identification demonstrated no evidence of somaclonal variation as a result of cryopreservation. The results of this work indicate the applicability of cryopreservation as a long-term storage strategy for spruce embryogenic cultures from a wide genetic background.
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Abbreviations
- ABA:
-
± abscisic acid
- BA:
-
N6-benzyladenine
- BSA:
-
bovine serum albumin
- CTAB:
-
cetyldimethylethyl-ammonium bromide
- DMSO:
-
dimethylsulfoxide
- DTT:
-
dithiothreitol
- EDTA:
-
ethylenediaminetetraacetic acid disodium salt
- IBA:
-
indole-3-butyric acid
- LN:
-
liquid nitrogen
- PEG:
-
polyethylene glycol
- SLS:
-
N-lauroyl sarcosine
- Tris:
-
tris[hydroxymethylamino] methane
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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Communicated by B. E. Ellis
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Cyr, D.R., Lazaroff, W.R., Grimes, S.M.A. et al. Cryopreservation of interior spruce (Picea glauca engelmanni complex) embryogenic cultures. Plant Cell Reports 13, 574–577 (1994). https://doi.org/10.1007/BF00234514
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DOI: https://doi.org/10.1007/BF00234514