Summary
Hairy root cultures of Hyoscyamus muticus were established using Agrobacterium rhizogenes ATCC 15834. In one out of 8 clones established, an unusual root tip formation was observed after transfer of cultures from half-strength Murashige and Skoog (1962) to White's medium (1939). This phenomenon was associated with the production of a fine brownish cell suspension culture. Hairy root development resumed after transfer of the root tips from White to half-strength Murashige and Skoog medium. After plating the isolated brownish cells on hormone-free half-strength Murashige and Skoog or White solid medium, callus proliferation was observed, and then redifferentiation of hairy roots occurred. The polymerase chain reaction analysis of the H. muticus hairy root (clone Z2) revealed that only the tl region of the T-DNA was integrated. The growth and the production of five tropane alkaloids by this clone were examined.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PCR:
-
Polymerase Chain Reaction
- MS medium:
-
Murashige and Skoog Medium
- 1/2 MS medium:
-
half-strength MS medium
- WP medium:
-
Woody Plant medium
- RC medium:
-
Root Culture medium
- WH medium:
-
White medium
- HPLC:
-
High Performance Liquid Chromatography
- wt.:
-
weight
References
Bailey J A (1982) In: Baily J A, Mansfield J W (ed) Phytoalexins, Blackie, Glasgow and London, pp 289–318.
Darvill A G, Albersheim P, Bucheli P, Doares S, Doubrava N, Eberhard S, Gollin D J, Hahn M G, Marfê-Riera V, York W S, Mohnen D (1989) In: Ben J. J. Lugtenberg (ed) Signal molecules in plant and plant microbe interactions, Nato ASI Series H: Cell biology, vol 36. Springer-Verlag, Berlin Heidelberg New York, pp 41–48.
Edwards K, Johnstone C, Thompson C (1991) Nucleic Acids Research 19: 1349.
EMBO course (1982) The use of Ti plasmid as cloning vector for genetic engineering in plants, August 4–23, p. 109.
Flores H E, and Filner P, (1985) In: Neuman K H, Barz W, Reinhard E (ed) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York Tokyo, pp 174–185.
Fujita Y, Hara Y, Suga C, Morimoto T (1981) Plant Cell Rep. 1: 61–63.
Jouanin L (1984) Plasmid 12: 91–102.
Lloyd G B, Mc.Cown B H (1980) Inter. Plant Prop. Soc. 30: 421–423.
Murashige T, and Skoog F, (1962) Physiol. Plant 15: 473–497.
Petit A, David C, Dahl, G A, Ellis, J G, Guyon, P, Casse-Delbart, F, Tempé J (1983) Mol. Gen. Genet. 190, 204–214
Rhodes M J C, Robins R J, Hamil J D, Parr A J, Hilton M G, Walton N J (1990) In: Charlwood B V, Rhodes MJC (ed) Secondary Products from Plant Tissue Culture. Clarendon Press, Oxford, pp 201–226.
Saiki R K, Gelfand D H, Stoffel S, Scharf S J, Higuchi R, Horn G, Mullis K B, Erlich H A (1988) Science 239: 487–491.
Sauerwein M, Shimomura K (1991) Phytochem. 30: 2275–2279.
Shimomura K, Sauerwein M, Ishimaru K (1991) Phytochem. 30: 2275–2279.
Slightom J L, Durant-Tardif M, Jouanin L, Tepfer D (1982) Journal of Biological Chemistry 261: 108–121.
Strauss A, (1989) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 7. Medicinal and aromatic plants II. Springer-Verlag, Berlin Heidelberg New York London Paris Tokyo, pp 286–314.
Tabata M, Fujita Y (1985) In: Zaitlin M, Hollaender A (ed) Biotechnology in Plant Sciences. Relevance of Agriculture in the Eighties. Academic Press, Orlando, pp 207–218.
Vaughan D, Ord B (1990) J. Sci. Food Agri. 52: 289–299.
White P R (1939) Am. J. Bot. 26: 59–64.
Author information
Authors and Affiliations
Additional information
Communicated by A. Komamine
Rights and permissions
About this article
Cite this article
Jaziri, M., Homes, J. & Shimomura, K. An unusual root tip formation in hairy root culture of Hyoscyamus muticus . Plant Cell Reports 13, 349–352 (1994). https://doi.org/10.1007/BF00232635
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232635