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Transgenic Brassica napus plants obtained by cocultivation of protoplasts with Agrobacterium tumefaciens

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Abstract

Hypocotyl protoplasts of German winter oilseed, rape (Brassica napus) lines of double-low quality were transformed using Agrobacterium tumefaciens harbouring pGV 3850∶1103 neo (dimer) containing chimaeric kanamycin resistance reporter genes. Transformed protoplasts were regenerated to fertile and phenotypically normal plants. Transformation was confirmed by kanamycin resistance, nopaline production, neomycinphosphotransferase II activity, and Southern blot hybridization. Seed progeny from self-pollinated transformants expressed the introduced kanamycin resistance as a Mendelian trait.

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Abbreviations

BAP:

6-benzylaminopurine

Cf:

ClaforanR

2.4D:

2,4-dichlorophenoxy acetic acid

Km:

kanamycin

MS:

Murashige and Skoog (1962)

NAA:

α-naphthalene acetic acid

NPT II:

neomycinphosphotransferase

npt II:

neomycinphosphotransferase II gene

NOS:

nopaline synthase

nos:

nopaline synthase gene

ocs:

octopine synthase gene

IAA:

indole-3-acetic acid

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Authors and Affiliations

  1. Bayer AG, Agrochemicals Division, Development/Biotechnology, D-5090, Leverkusen-Bayerwerk, Federal Republic of Germany

    J. E. Thomzik & R. Hain

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  1. J. E. Thomzik
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  2. R. Hain
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Communicated by H. Lörz

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Thomzik, J.E., Hain, R. Transgenic Brassica napus plants obtained by cocultivation of protoplasts with Agrobacterium tumefaciens . Plant Cell Reports 9, 233–236 (1990). https://doi.org/10.1007/BF00232290

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  • DOI: https://doi.org/10.1007/BF00232290

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