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Alterations in cardiac membrane Ca2+ transport during oxidative stress

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Abstract

Although cardiac dysfunction due to ischemia-reperfusion injury is considered to involve oxygen free radicals, the exact manner by which this oxidative stress affects the myocardium is not clear. As the occurrence of intracellular Ca2+ overload has been shown to play a critical role in the genesis of cellular damage due to ischemia-reperfusion, this study was undertaken to examine whether oxygen free radicals are involved in altering the sarcolemmal Ca2+-transport activities due to reperfusion injury. When isolated rat hearts were made globally ischemic for 30 min and then reperfused for 5 min, the Ca2+ -pump and Na+-Ca2+ exchange activities were depressed in the purified sarcolemmal fraction; these alterations were prevented when a free radical scavenger enzymes (superoxide dismutase plus catalase) were added to the reperfusion medium. Both the Ca2+- pump and Na+- Ca2+ exchange activities in control heart sarcolemmal preparations were depressed by activated oxygen-generating systems containing xanthine plus xanthine oxidase and H2O2; these changes were prevented by the inclusion of superoxide dismutase and catalase in the incubation medium. These results support the view that oxidative stress during ischemia-reperfusion may contribute towards the occurrence of intracellular Ca2+ overload and subsequent cell damage by depressing the sarcolemmal mechanisms governing the efflux of Ca2+ from the cardiac cell.

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Authors and Affiliations

  1. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, R2H 2A6, Winnipeg, Manitoba, Canada

    Ian M. C. Dixon, Masanori Kaneko, Tomoji Hata, Vincenzo Panagia & Naranjan S. Dhalla

  2. Department of Physiology, Faculty of Medicine, University of Manitoba, R2H 2A6, Winnipeg, Manitoba, Canada

    Ian M. C. Dixon, Masanori Kaneko, Tomoji Hata, Vincenzo Panagia & Naranjan S. Dhalla

Authors
  1. Ian M. C. Dixon
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  2. Masanori Kaneko
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  3. Tomoji Hata
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  4. Vincenzo Panagia
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  5. Naranjan S. Dhalla
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Dixon, I.M.C., Kaneko, M., Hata, T. et al. Alterations in cardiac membrane Ca2+ transport during oxidative stress. Mol Cell Biochem 99, 125–133 (1990). https://doi.org/10.1007/BF00230342

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