Skip to main content
SpringerLink
Log in

Responses of alfalfa pollen and callus to filtrates from two isolates of Fusarium oxysporum

  • Original Article
  • Published:
Sexual Plant Reproduction Aims and scope Submit manuscript

Summary

For 25 Medicago sativa plants, measurements were made of in vitro pollen germination and growth and callus growth on mediums containing culture filtrate from two isolates of Fusarium oxysporum f. sp. medicaginis. In vivo resistance was determined by field inoculation with one isolate. There was no correlation between any in vitro measurement and in vivo resistance, and none of the in vitro measurements on control mediums were correlated. The only significant correlation for the same measurement between the two isolates was for pollen-tube length (r = 0.65). Percent of pollen germination was negatively correlated with callus growth for both isolates. Earlier work showed that callus growth in the presence of culture filtrate was linked to plant resistance, thus it appeared that percent pollen germination on culture filtrate had decreased for the more resistant plants. The haploid pollen may be used as a progeny test for identifying heterotic loci conferring resistance to Fusarium, but if the pollen of this plant species is used in direct selection by exposure to culture filtrate, the level of resistance to Fusarium may decrease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barnes DK, Cleveland RW (1963) Pollen-tube growth of diploid alfalfa in vitro. Crop Sci 3:291–295

    Google Scholar 

  • Bell AA, Mace ME (1980) Biochemistry and physiology of resistance. In: Mace ME, Bell AA, Beckman CH (eds) Fungal wilt disease of plants. Academic Press, New York, pp 431–486

    Google Scholar 

  • Buiatti M, Sclala A, Bettini P, Nascari G, Morpurgo R, Bogani P, Pellegrini P, Gimelli G, Venturo R (1985) Correlations between in vitro resistance to Fusarium and in vitro response to fungal elicitors and toxic substances in carnation. Theor Appl Genet 70:42–47

    Google Scholar 

  • Frosheiser FI, Barnes DK (1978) Field reaction of artificially inoculated alfalfa populations to the Fusarium and bacterial wilt pathogens alone and in combination. Phytopathology 68:943–946

    Google Scholar 

  • Hartman CL, McCoy TJ, Knous TR (1984) Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced by Fusarium oxysporum f. sp. medicaginis. Plant Sci Lett 34:183–194

    Google Scholar 

  • Helgeson JP (1983) Studies of host-pathogen interaction in vitro. In: Helgeson JP, Deverall BJ (eds) Use of tissue culture and protoplast in plant pathology. Academic Press, Sydney, pp 9–38

    Google Scholar 

  • Ottaviano E, Mulcahy DL (1989) Genetics of angiosperm pollen. Adv Genet 6:1–64

    Google Scholar 

  • Rajora OP, Zsuffa L (1986) Sporophytic and gametophytic gene expression in Populus deltoides Marsh, P nigra L., and P. maximowiczii Henry. Can J Genet Cytol 28:476–482

    Google Scholar 

  • Sacristan MD (1986) Isolation and characterization of mutant cell lines and plants: disease resistance. In: Vasil IK (ed) Cell culture and somatic cell genetics, vol 3: plant regeneration and genetic variability. Academic Press, New York, pp 512–525

    Google Scholar 

  • Sari-Gorla, Frova C, Binelli G, Ottaviano E (1986) The extent of gametophytic-sporophytic gene expression in maize. Theor Appl Genet 72:42–47

    Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204

    CAS  Google Scholar 

  • Shivanna KR, Linskens HF, Cresti M (1991) Pollen viability and pollen vigor. Theor Appl Genet 81:38–42

    Google Scholar 

  • Simon CJ, Sanford JC (1986) Induction of gametic selection in situ by stylar application of selective agents. In: Mulcahy D, Mulcahy GB, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, Berlin Heidelberg New York, pp 107–112

    Google Scholar 

  • Tanksley SD, Zamir D, Rick CM (1981) Evidence for extensive overlap of sporophytic and gametophytic gene expression in Lycopersicon esculentum. Science 231:453–455

    Google Scholar 

  • Willing RP, Mascarenhas JP (1984) Analysis of the complexity and diversity of mRNA's from pollen and shoots of Tradescantia. Plant Physiol 74:865–868

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. USDA-ARS, Crop Science Laboratory, 39762, Mississippi State, MS, USA

    D. E. Rowe

  2. Department of Plant Sciences, University of Nevada, 89557, Reno, NV, USA

    D. L. Stortz-Lintz

Authors
  1. D. E. Rowe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. L. Stortz-Lintz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rowe, D.E., Stortz-Lintz, D.L. Responses of alfalfa pollen and callus to filtrates from two isolates of Fusarium oxysporum . Sexual Plant Reprod 6, 11–15 (1993). https://doi.org/10.1007/BF00227577

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00227577

Key words

Search

Navigation