Summary
The endosperm proteins encoded by the genome Hch in Hordeum chilense, Tritordeum (amphiploid Hordeum chilense x Triticum turgidum), common wheat-H. chilense addition lines, and the segregating plants resulting from the cross Tritordeum x T. turgidum, were fractionated by three electrophoretical techniques: SDS-PAGE, A-PAGE, and bidimensional PAGE. Prolamin subunits with a high molecular weight (HMW) were well visualized by SDS-PAGE, the A-PAGE technique permitted good resolution for many hordeins and gliadins, and two-dimensional electrophoresis allowed new sets of bands coded by gene complexes from H. chilense chromosomes to be distinguished. The loci Hor-Hch1 (up to 11 subunits belonging to the ω-, β — and α-hordeins), Glu-Hch1 (one HMW prolamin subunit), Hor-Hch2 (one α-hordein), and Hor-Hch3 (up to four α-hordeins) were located on the H. chilense chromosomes 1Hch, 5Hch, and 7Hch.
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References
Ainsworth CC, Gale MD, Baird S (1984) The genetic control of grain esterases in hexaploid wheat. 1. Allelic variation. Theor Appl Genet 8:219–226
Ainsworth CC, Gale MD, Miller TE (1986) Genetic control of grain esterases in hexaploid wheat. 2. Homoeologous loci in related species. Theor Appl Genet 72:219–225
Benito C, Frade JM, Orellana J, Carrillo JM (1990) Linkage and cytogenetic maps of genes controlling endosperm storage proteins and isozymes in rye (Secale cereale L.). Theor Appl Genet 79:347–352
Chapman V, Miller TE (1978) The amphiploid of Hordeum chilense (x) Triticum aestivum. Cereal Res Commun 6:351–352
Chojecki AJS, Gale MD (1982) Genetic control of glucose phosphate isomerase in wheat and related species. Heredity 49:337–347
Fernández JA (1989) Genetics of Hordeum chilense — current status. Plant Breed 102:10–16
Fernández JA, Jouve N (1987a) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 1. 6-phosphogluconate dehydrogenase and malate dehydrogenase. Theor Appl Genet 73:433–439
Fernández JA, Jouve N (1987b) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 3. Esterases, glutamate oxaloacetate transaminase, and phosphoglucomutase. Theor Appl Genet 73:690–698
Fernández JA, Jouve N (1988) The addition of Hordeum chilense chromosomes to Triticum turgidum conv. durum. Biochemical, karyological, and morphological characterization. Euphytica 37:247–259
Fernández JA, Jouve N (1990) Chromosomal location of genes encoding a leaf phosphatase and a cathodal peroxidase in the wild barley Hordeum chilense. J Hered (in press)
Galili G, Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193:293–298
Lafiandra D, Kasarda D (1985) One- and two-dimensional (two-pH) polyacrylamide gel electrophoresis in a single gel separation of wheat proteins. Cereal Chem 62:314–319
Martin A, Chapman V (1977) A hybrid between Hordeum chilense and Triticum aestivum. Cereal Res Commun 9:317–323
Martin A, Cubero JI (1981) The use of Hordeum chilense in cereal breeding. Cereal Res Commun 9:317–323
Martiń A, Sánchez-Monge Laguna E (1982) Cytology and morphology of the amphiploid Hordeum chilense x Triticum turgidum conv. durum. Euphytica 31:261–267
Martín A, Sánchez-Monge Laguna E (1985) Effects of the 5B system on control of pairing in Hordeum chilense x Triticum aestivum hybrids. Z Pflanzenzuecht 85:122–127
Miller TE, Reader SM, Chapman V (1982) The addition of Hordeum chilense chromosomes to wheat. Induced variability in plant breeding. Eucarpia Int Symp, 1981. Pudoc, Wageningen, pp 79–81
Miller TE, Reader SM, Ainsworth CC (1985) A chromosome of Hordeum chilense homoeologous to group 7 of wheat. Can J Genet Cytol 27:101–104
Payne PI, Corfield KG, Holt LM, Blackman JA (1981) Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progeny of six crosses of bread wheat. J Sci Food Agric 32:51–60
Payne PI, Jackson LM, Holt LM, Law CN (1984) Genetic linkage between endosperm protein genes of each of the short arms of chromosomes 1A and 1B in wheat. Theor Appl Genet 67:235–243
Payne PI, Holt LM, Reader SM, Miller TE (1987) Chromosomal location of genes coding for endosperm proteins of Hordeum chilense determined by two-dimensional electrophoresis of -wheat/H. chilense chromosome addition lines. Biochem Genet 25:53–65
Shewry PR, Bradberry D, Franklin J, White RP (1984) The chromosomal locations and linkage relationships of the structural genes for prolamin storage proteins (secalins) of rye. Theor Appl Genet 69:63–69
Shewry PR, Tatham AS, Field JM, Forde BC, Clarck J, Gallois P, Marris C, Halford M, Forde J, Kreis M (1988) The structures of barley and wheat prolamins and their genes. Biochem Physiol Pflanz 183:117–127
Sybenga J, Parmar S, Eden J van, Shewry P (1990) Mapping seed storage protein loci Sec-1 and Sec-3 in relation to five chromosomal rearrangements in rye (Secale cereale L.). Theor Appl Genet 79:201–208
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Commicated by J. Mac Key
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Tercero, J.A., Bernardo, A. & Jouve, N. Encoding genes for endosperm proteins in Hordeum chilense . Theoret. Appl. Genetics 81, 127–132 (1991). https://doi.org/10.1007/BF00226122
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DOI: https://doi.org/10.1007/BF00226122