Summary
Total DNA from callus tissue of 28 accessions representing seven wild perennial Glycine species was compared using recombinant genomic probes derived from G. max, the soybean. Using two probes, we show that this molecular approach both confirms and extends the model for the taxonomic relationships between the species derived from morphological and cytogenetic data, and that it provides clear evidence that RFLP analysis of genomic sequences has the potential for revealing the derivation of the member species of the wild perennial Glycine taxon. Although, in this preliminary report, the sample size for each species is small, it is clear that the greatest between-accession variation occurs in G. tabacina (B2B2) and G. clandestine (A1A1), suggesting that these may be the taxa from which further speciation occurred in the subgenus.
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References
Bouché JP (1981) The effect of spermidine on endonuclease inhibition by agarose contaminants. Anal Biochem 115:2–45
Broué P, Marshall DR, Muller WJ (1977) Biosystematics of subgenus Glycine Verde.: isoenzymic data. Aust J Bot 25:355–356
Burr BA, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in Maize. Genetics 118:519–526
Constanza SH, Hymowitz T (1987) Adventitious roots in Glycine subg. Glycine Leguminosae: morphological and taxonomic indicators of the B genome. Plant Syst Evol 158:37–46
Denhardt D (1966) Detection of specific sequences among fragments separated by gel electrophoresis. Biochem Biophys Res Commun 23:641–646
Doyle JJ (1987) Variation at the DNA level: uses and potential in legume systematics. In: Stirton CH (ed) Advances in legume systematics. Royal Botanic Gardens, Kew, England, pp 1–30
Doyle JJ, Beachy RN (1985) Ribosomal gene variation in soybean (Glycine) and its relatives. Theor Appl Genet 70: 369–376
Doyle JJ, Brown AHD (1989) 5S nuclear ribosomal gene variation in the Glycine tomentella polyploid complex. Syst Bot 14:621–624
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Feinberg AP, Vogelstein B (1984) Addendum. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5:109–118
Hymowitz T, Singh RJ (1987) Taxonomy and speciation. In: Wilcox JR (ed) Soybeans: improvement, production, and uses. Agron Monogr vn16: 23–48
Krueger RW, Holland MA, Chisholm D, Polacco JC (1987) Recovering soybean urease genomic clone by sequential library screening with two synthetic oligonucleotides. Gene 54:41–50
Larkin PJ, Scowcroft WR (1983) Somaclonal variation and crop improvement. In: Kosuge T, Meredith CP, Hollander A (eds) Genetic engineering in plants. Plenum Press, New York, pp 289–314
Lee M, Phillips RL (1987) Genomic rearrangements in maize induced by tissue culture. Genome 29:122–128
Menancio DI (1988) Isoenzyme and RFLP studies in the genus Glycine Willd., subgenus Glycine. PhD thesis, University of Ilinois at Urbana-Champaign
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Newell CA, Hymowitz T (1978) A reappraisal of the subgenus Glycine. Am J Bot 65:168–179
Newell CA, Hymowitz T (1983) Hybridization in the genus Glycine subgenus Glycine Willd. (Leguminosae, Papilionoideae). Am J Bot 70:334–348
Phillips GC, Collins GB (1979) In vitro tissue culture of selected legumes and plant regeneration from callus subcultures of red clover. Crop Sci 19:59–64
Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221
Singh RJ (1986) Chromosomal variation in immature embryo-derived calluses of barley (Hordeum vulgare L.). Theor Appl Genet 72:710–716
Singh RJ, Hymowitz T (1985) The genomic relationships among six wild perennial species of the genus Glycine subgenus Glycine Willd. Theor Appl Genet 71:221–230
Singh RJ, Kollipara KP, Hymowitz T (1987) Intersubgeneric hybridization of soybeans with a wild perennial species, Glycine clandestina Wendl. Theor Appl Genet 74:391–396
Singh RJ, Kollipara KP, Hymowitz T (1988) Further data on the genomic relationships among wild perennial species (2n=40) of the genus Glycine Willd. Genome 30:166–176
Tindale MD (1984) Two new Eastern Australian species of Glycine Willd. (Fabaceae). Brunonia 7:207–312
Tindale MD, Craven LA (1988) Three new species of Glycine (Fabaceae: Phaseolae) from North-Western Australia with notes on amphicarpy in the genus. Aust Syst Bot 1:399–410
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Communicated by A. L. Kahler
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Menancio, D.I., Hepburn, A.G. & Hymowitz, T. Restriction fragment length polymorphism (RFLP) of wild perennial relatives of soybean. Theoret. Appl. Genetics 79, 235–240 (1990). https://doi.org/10.1007/BF00225957
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DOI: https://doi.org/10.1007/BF00225957