Summary
A mouse monoclonal antibody was prepared by using homogenized fragments of crude umbrella material of the hydromedusa Podocoryne carnea as an antigen. The selected clone produced an IgG (mAb sm-1) which decorated smooth muscle cells of hydrozoans. Immunohistochemical testing of mAb sm-1 on whole-mount preparations revealed reactivity with a cytoplasmic, formaldehyde-resistant antigen present in the smooth muscle cells, but absent in all other cell-types. The antibody can therefore be used as a selective and highly sensitive marker to trace the pattern of the smooth muscle system in hydrozoans. The tight association between smooth muscle cells and nerve cells which show FMRFamide-like immunoreactivity can be demonstrated in whole-mount preparations of the hydromedusa Podocoryne carnea with a polyclonal anti-FMRFamide antiserum and in double-labelling experiments.
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Weber, C. Smooth muscle fibers of Podocoryne carnea (Hydrozoa) demonstrated by a specific monoclonal antibody and their association with neurons showing FMRFamide-like immunoreactivity. Cell Tissue Res. 255, 275–282 (1989). https://doi.org/10.1007/BF00224109
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DOI: https://doi.org/10.1007/BF00224109