Summary
Protoplasts derived from different cytoplasmic male-sterile cultivars of Nicotiana tabacum were fused. Nearly 200 cybrid calli were regenerated into plants and their flower morphologies were examined. Most cybrids exhibited parental-type male-sterile morphologies. Some, however, showed novel male-sterile phenotypes or phenotypes which combined traits of both male-sterile parents in a new combination. Others were restored to fertility, with stamens which produced functional pollen.
Similar content being viewed by others
References
Aviv D, Galun E (1980) Restoration of fertility in cytoplasmic male-sterile (CMS) Nicotiana sylvestris by fusion with X-irradiated N. tabacum protoplasts. Theor Appl Genet 58:121–127
Aviv D, Galun E (1986) Restoration of male-fertile Nicotiana by fusion of protoplasts derived from two different cytoplasmic male-sterile cybrids. Plant Mol Biol 7:411–417
Aviv D, Fluhr F, Edelman M, Galun E (1980) Progeny analysis of the interspecific somatic hybrids: Nicotiana tabacum (CMS) and Nicotiana sylvestris with respect to nuclear and chloroplast markers. Theor Appl Genet 56:145–150
Belliard G, Pelletier G, Vedel F, Quetier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Mol Gen Genet 165:231–237
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Caboche M (1980) Nutritional requirements of protoplast derived haploid tobacco cells grown at low cell densities in liquid medium. Planta 149:7–18
Gerstel, DU (1980) Cytoplasmic male sterility in Nicotiana (a review). NC Agric Res Serv Tech Bull 263:1–31
Gleba YY (1978) Extranuclear inheritance investigated by somatic hybridization. In: Thorpe TA (ed) Frontiers of plant tissue culture. Int Assoc for Plant Tissue Culture, Calgary, pp 95–102
Glimelius K, Bonnett HT (1981) Somatic hybridization in Nicotiana: Restoration of photoautotrophy to an albino mutant with defective plastids. Planta 153:497–503
Glimelius K, Wallin A, Eriksson T (1978) Concanavalin A improves the polyethylene glycol method for fusing plant protoplasts. Physiol Plant 44:92–96
Glimelius K, Chen K, Bonnett HT (1981) Somatic hybridization in Nicotiana: Segregation of organellar traits among hybrid and cybrid plants. Planta 153:504–510
Glimelius K, Djupsjöbacka M, Fellner-Feldegg H (1986) Selection and enrichment of plant protoplast heterokaryons of Brassicaceae by flow sorting. Plant Sci 45:133–141
Hein T, Przewozny T, Schieder O (1983) Culture and selection of somatic hybrids using an auxotrophic cell line. Theor Appl Genet 64:119–122
Johansson L, Andersson B, Eriksson T (1982) Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO2 concentration. Physiol Plant 54:24–30
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110
Lonsdale DM, Leaver CJ (1988) Mitochondrial gene nomenclature. Plant Mol Biol Rep 6:14–21
Menczel L, Nagy T, Kiss ZsR, Maliga P (1981) Streptomycin-resistant and sensitive somatic hybrids of Nicotiana tabacum and Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Theor Appl Genet 59:191–195
Müller AJ, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nagy JI, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Z Pflanzenphysiol 78:453–455
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87
Pelletier GR (1986) Plant organelle genetics through somatic hybridization. Oxford Surv Plant Mol Cell Biol 3:97–121
Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Pousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191:244–250
Rosenberg SM, Bonnett HT (1983) Floral organogenesis in Nicotiana tabacum: A comparison of two cytoplasmic male-sterile cultivars with a male-fertile cultivar. Am J Bot 70:266–275
Sundberg E, Glimelius K (1986) A method for production of interspecific hybrids within Brassicaceae via somatic hybridization using resynthesis of Brassica napus as a model. Plant Sci 43:155–162
Author information
Authors and Affiliations
Additional information
Communicated by P. Maliga
Rights and permissions
About this article
Cite this article
Kofer, W., Glimelius, K. & Bonnett, H.T. Modifications of floral development in tobacco induced by fusion of protoplasts of different male-sterile cultivars. Theoret. Appl. Genetics 79, 97–102 (1990). https://doi.org/10.1007/BF00223793
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223793