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Ultrastructure of mucosal mast cells in normal and compound 48/80-treated rats

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Summary

Mast cells in the tongue and lamina propria of the duodenal mucosa, representing two cell types with different morphological, histochemical and functional properties have been studied under the electron microscope. Both cell types were found to contain similar moderately electron dense and homogeneous granules surrounded by a tight membrane constituting the basic ultrastructural characteristics of mast cells. The mucosal mast cells, however, contained fewer granules per cell and the individual granules often exhibited considerable variation in size. In addition the mucosal mast cells, unlike those of the tongue, peritoneum and skin, had a smooth plasma membrane lacking microvilli and possessed irregularly shaped or lobulated nuclei. In the tongue mast cells, administration of Compound 48/80 caused the formation of vacuoles around the granules, expulsion of granules from the cells and disintegration of the granular matrix, but no changes that could be attributed to 48/80 could be found in the mucosal mast cells.

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References

  • Bloom, G., Haegermark, Ö.: A study on morphological changes and histamine release induced by Compound 48/80 in rat peritoneal mast cells. Exp. Cell Res. 40, 637–654 (1965)

    Google Scholar 

  • Bloom, G., Larsson, B., Åberg, B.: Canine mastocytoma. Zbl. Vet.-Med. 5, 443–458 (1958)

    Google Scholar 

  • Boeryd, B., Lundin, P. M., Norrby, K., Schelin, U.: Ultrastructure of a mouse sarcoma in its solid, enzymatically dissociated and ascitic forms. Int. J. Cancer 3, 283–290 (1968)

    Google Scholar 

  • Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. II. Dyebinding and metachromatic properties. Acta path, microbiol. scand. 66, 303–312 (1966a)

    Google Scholar 

  • Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. III. Reactivity towards compound 48/80. Acta path, microbiol. scand. 66, 313–322 (1966c)

    Google Scholar 

  • Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. IV. Monoamine storing capacity. Acta path, microbiol. scand. 67, 365–379 (1966b)

    Google Scholar 

  • Enerbäck, L., Häggendahl, J.: Uptake and storage of catecholamines in mucosal mast cells of the rat. J. Histochem. Cytochem. 18, 803–811 (1970)

    Google Scholar 

  • Enerbäok, L., Löwenhagen, G. B.: Long term increase in mucosal mast cells of the rat induced by compound 48/80. (To be published)

  • Enerbäck, L., Löwenhagen, G. B., Löwenhagen, O.: Effect of polymyxin B and some mast cell constituents on the quantity of mucosal mast cells in the rat. (To be published)

  • Fernando, N. V., Movat, H. Z.: The fine structure of connective tissue. III. The mast cell. Exp. molec. Path. 2, 450–463 (1963)

    Google Scholar 

  • Hibbs, R. G., Burch, G. E., Phillips, J. H.: Electron-microscopic observations on the human mast cell. Amer. Heart J. 60, 121–127 (1960)

    Google Scholar 

  • Högberg, B., Uvnäs, B.: The mechanism of the disruption of mast cells produced by compound 48/80. Acta physiol. scand. 41, 345–369 (1957)

    Google Scholar 

  • Krüger, P. G., Bloom, G. D.: Granule alteration and vacuole formation, Two primary structural features of the histamine release process in mast cells. Experientia (Basel) 29, 329–330 (1973)

    Google Scholar 

  • Lagunoff, D.: Contributions of electron microscopy to the study of mast cells. J. invest. Derm. 58, 296–311 (1972)

    Google Scholar 

  • Michels,N. A.: The mast cell. In: Handbook of haematology. Ed. by H. Downey, I, p. 232–372. London (1938). Eepublished in: Ann. N.Y. Acad. Sci. 103, Appendix 232–372 (1963)

  • Miller, H.R.P., Jarret, W.F.H.: Immune reactions in mucous membranes. I. Intestinal mast cell response during helminth expulsion in the rat. Immunology 20, 277–288 (1971)

    Google Scholar 

  • Miller, H.R.P., Walshaw, R.: Immune reactions in mucous membranes. IV. Histochemistry of intestinal mast cells during helminth expulsion in the rat. Amer. J. Path. 69, 195–208 (1972)

    Google Scholar 

  • Mota, I.: Mast cells and anaphylaxis. Ann. N.Y. Acad. Sci. 103, 264–277 (1963)

    Google Scholar 

  • Mota, I., Beraldo, W., Ferri, A. G., Junqueira, L.C.U.: Action of 48/80 on the mast cell population and histamine content of the wall of the gastrointestinal tract of the rat. Ciba Foundation symposium on histamine. London: J. and A. Churchill Ltd. 1956

    Google Scholar 

  • Murray, M., Miller, H.R.P., Jarret, W.F.H.: The globule leucocyte and its derivation from the subepithelial mast cell. Lab. Invest. 19, 222–234 (1968)

    Google Scholar 

  • Riley, J. F.: The mast cells. Edinburgh and London: E & S. Livingstone Ltd. 1959

    Google Scholar 

  • Riley, J. F., West, G. B.: Tissue mast cells. Studies with a histamine liberator with low toxicity (compound 48/80). J. Path. Bact. 69, 269–282 (1955)

    Google Scholar 

  • Röhlich, P., Anderson, P., Uvnäs, B.: Electron microscope observations on compound 48/80-induced degranulation in rat mast cells. J. Cell Biol. 61, 465–483 (1971)

    Google Scholar 

  • Rogers, G. E.: Electron microscopy of mast cells in the skin of young mice. Exp. Cell Res. 11, 393–402 (1956)

    Google Scholar 

  • Singleton, E. M., Clark, S. L.: The response of mast cells to compound 48/80 studied with the electron microscope. Lab. Invest. 14, 1744–1763 (1965)

    Google Scholar 

  • Smith, D. E.: Electron microscopy of normal mast cells under various experimental conditions. Ann. N.Y. Acad. Sci. 103, 40–52 (1963)

    Google Scholar 

  • Uvnäs, B., Thon, I.-L.: Evidence for enzymatic histamine release from isolated rat mast cells. Exp. Cell Res. 23, 45–57 (1961)

    Google Scholar 

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Supported by grants from the Swedish Medical Research Council (Project 14X-2235).

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Enerbäck, L., Lundin, P.M. Ultrastructure of mucosal mast cells in normal and compound 48/80-treated rats. Cell Tissue Res. 150, 95–105 (1974). https://doi.org/10.1007/BF00220383

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  • DOI: https://doi.org/10.1007/BF00220383

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