Summary
Pituitary somatotrops were localized in four species from the genus Oncorhynchus using an antiserum to chum salmon (Oncorhynchus keta) growth hormone. The antiserum cross-reacted specifically with the acidophils in the caudal pars distalis of all species tested. Corroborative immunodiffusion studies were also conducted with pituitary homogenates from the same species. Immunological identity of the GH molecule was observed for all species tested.
References
Ball JN, Baker BI (1969) The pituitary gland: anatomy and histophysiology. In: Hoar WS, Randall DJ (eds) Fish physiology, Vol 2, Academic Press, New York, pp 1–110
Chadwick AC, Oreg FB, Tai-Chang S (1976) Immunofluorescent characterization of pituitary cells in different vertebrate groups. Gen Comp Endocrinol 29:265–265
Graham BC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Hansen GN, Hansen BL (1975) Immunocytochemical localization of growth hormone and prolactin in the pituitary of Acipenser güldenstädti Brandt (Chondrostei). Acta Zool 56:29–41
Ingleton PM, Stribley MF (1975) Immunofluorescent identification of the cells of origin of teleost pituitary hormones separated by polyacrylamide gel electrophoresis. J Endocrinol 67:41
Ingleton PM, Stribley MF (1977) Immunofluorescent identification of the cells of origin of Eel (Anguilla anguilla) pituitary hormones separated by polyacrylamide gel electrophoresis. Gen Comp Endocrinol 31:37–44
Komourdjian MP, Idler DR (1979) Chum salmon pituitary fractions: somatotrophic activity and cytoimmunoflourescent studies. Gen Comp Endocrinol 37:343–349
McKeown BA, van Overbeeke AP (1971) Immunohistochemical identification of pituitary hormone producing cells in the sockeye salmon (Oncorhynchus nerka, Walbaum). Z Zellforsch 112:350–362
Nagahama Y, Olivereau M, Farmer SW, Nishioka RS, Bern HA (1981) Immunocytochemical identification of the prolactin and growth hormone-secreting cells in the teleost pituitary with antisera to Tilapia prolactin and growth hormone. Gen Comp Endocrinol 44:339–395
Ouchterlony O (1968) Handbook of immunodiffusion and immunoelectrophoresis. Ann Arbour Science Publishers, Ann Arbour, Michigan, USA
Sternberger LA (1974) Immunocytochemistry, Prentice-Hall, Englewood Cliffs, pp 246
Vaitukaitus J, Robbins JB, Nieschlag E, Ross GT (1972) A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol 33:988–991
Wagner GF, McKeown BA (1983) The purification, partial characterization and bioassay of growth hormone from two species of Pacific salmon. In: Lofts B (ed) Comparative endocrinology. Proceedings of the 9th International Symposium on Comparative Endocrinology, Hong Kong 1981. University of Hong Kong Press (In Press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wagner, G.F., McKeown, B.A. The immunocytochemical localization of pituitary somatotrops in the genus Oncorhynchus using an antiserum to growth hormone of chum salmon (Oncorhynchus keta). Cell Tissue Res. 231, 693–697 (1983). https://doi.org/10.1007/BF00218126
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218126