Abstract
The presence of polygalacturonase and its correlation with the formation of lateral roots in leek (Allium porrum L.) seedlings have been investigated. During root growth, a steady increase in polygalacturonase activity was associated with that of the lateral root primordia. Fractionation of root extract by fast protein liquid chromatography resolved at least two polygalacturonase isoforms. One of the isoforms, a 75-kdalton protein, strongly reacted on Western blots probed with a polyclonal antibody raised against tomato polygalacturonase. It also reacted with both polyclonal and monoclonal antisera raised against Fusarium moniliforme polygalacturonase. In situ localization with these three antibodies showed that polygalacturonase was present over the meristems of lateral root primordia. Antibodies against pectins (Knox et al. 1990, Planta 181, 512–521) detected large amounts of pectic material filling the area between the apex of the primordium and the mother root tissues. We suggest that a polygalacturonase plays an important role in leek root morphogenesis, particularly during lateral root outgrowth.
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Abbreviations
- FPLC:
-
fast protein liquid chromatography
- RGU:
-
one unit of polygalacturonase activity
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
References
Ashford, A.E., McCully, M.E. (1970) Localization of naphtol ASB1 phosphatase activity in lateral and main root meristems of pea and corn. Protoplasma 70, 441–456
Ashford, A.E., McCully, M.E. (1973) Histochemical localization of β-glucosidases in roots of Zea mays. III. β-Glucosidase activity in the meristems of lateral roots. Protoplasma 77, 411–425
Berta, G., Fusconi, A., Trotta, A., Scannerini, S. (1990) Morphogenetic modifications by the mycorrhizal fungus Glomus strain E3 in the root system of Allium porrum L. New Phytol. 114, 207–215
Bonfante, P., Perotto, S. (1992) Plants and endomycorrhizal fungi: the cellular and molecular basis of their interaction. In: Molecular signals in plant-microbe communications, pp. 445–470, Verma, D.P.S., ed. CRC Press, Boca Raton, Fla., USA
Bonfante, P., Vian, B. (1989) Cell wall texture in symbiotic roots of Allium porrum L. Ann. Sci. Nat. Bot. 10, 97–109
Bonfante, P., Vian, B., Perotto, S., Faccio, A., Knox, J.P. (1990) Cellulose and pectin localization in roots of mycorrhizal Allium porrum: labelling continuity between host cell wall and interfacial material. Planta 180, 537–547
Bonfante, P., Tamagnone, L., Peretto, R., Esquerré-Tugayé, M.T., Mazau, D., Mosiniak, M., Vian, B. (1991) Immunocytochemical localizaton of hydroxyproline rich glycoproteins (HRGPs) at the interface zone between a mycorrhizal fungus and the host plants. Protoplasma 165, 127–138
Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248
Branca, C., De Lorenzo, G., Cervone, F. (1988) Competitive inhibition of the auxin-induced elongation by α-d-oligogalacturonides in pea stem segments. Physiol. Plant. 72, 499–504
Brown, S.M., Crouch, M.L. (1990) Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase. Plant Cell 2, 263–274
Cassab, G.I., Varner, J.E. (1989) Tissue printing on nitrocellulose paper: a new method for immunolocalization of proteins, localization of enzyme activities and anatomical analysis. Biol. Int. Rep. 13, 147–152
Charlton, W.A. (1991) Lateral root initiation. In: Plant roots: the hidden half, pp. 103–128, Waisel, Y., Eshel, A., Kafkafi, U., eds. Marcel Dekker Press, New York
Della Penna, D., Alexander, D.G., Bennett, A.B. (1986) Molecular cloning of tomato fruit polygalacturonase: analysis of polygalacturonase mRNA levels during ripening. Proc. Natl. Acad. Sci. USA 83, 6420–6424
De Lorenzo, G., Salvi, G., Degrà, R., D'Ovidio, R., Cervone, F. (1987) Induction of extracellular polygalacturonase and its mRNA in the phytopathogenic fungus Fusarium moniliforme. J. Gen. Microbiol. 133, 3365–3373
Estruch, J.L., Chriqui, D., Grossmann, K., Schell, J., Spena, A. (1991a) The plants oncogene rol C is responsible for the release of cytokinins from glucosyde conjugate. EMBO J. 10, 2889–2895
Estruch, J.L., Schell, J., Spena, A. (1991b) The protein encoded by the rol B plant oncogene hydrolyzes indole glucosydes. EMBO J. 10, 3125–3128
Filippini, F., Lo Schiavo, F., Terzi, M., Branca, C., Bellincampi, D., Salvi, G., Desiderio, A., De Lorenzo, G., Cervone, F. (1992) Phytoalexin elicitor-active α-1,4-d-oligogalacturonides reduce auxin perception by plant cells and tissues. In: Progress in plant growth regulation, pp. 517–521, Karssen, C.M., van Loon, L.C., Vrengdenhi, D., eds., Kluwer Academic Publishers, Amsterdam
Fischer, R.L., Bennett, A.B. (1991) Role of cell wall hydrolases in fruit ripening. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 675–703
Grierson, D., Tucker, G.A., Keen, J., Ray, J., Bird, C.R., Schuch, W. (1986) Sequencing and identification of a cDNA clone for tomato polygalacturonase. Nucleic Acids Res. 14, 8595–8603
Hahn, M.G., Bucheli, P., Cervone, F., Doares, S.H., O'Neill, R.A., Darvill, A., Albersheim, P. (1989) The role of the cell wall constituents in plant-pathogen interactions. In: Plant-microbe interactions, vol. 3, pp. 1–38, Nester, E., Kosuge, T., eds. McGraw-Hill, New York
Harlow, E., Lane, D. (1988) Antibodies. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
Hawes, M.C., Lin, H.-J. (1990) Correlation of pectolytic enzyme activity with the programmed release of cells from root caps of pea (Pisum sativum). Plant Physiol. 94, 1855–1859
Konno, H., Yamasaki, Y., Katoh, K. (1989) Extracellular exopolygalacturonase secreted from carrot cell cultures. Its purification and involvement in pectic polymer degradation. Physiol. Plant. 76, 514–420
Knox, J.P., Linstead, P.J., King, J., Cooper, C., Roberts, K. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta 181, 512–521
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 222, 680–685
Mauseth, J.D. (1988) Plant Anatomy. The Benjamin/Cummings Publishing Company, Menlo Park, Cal., USA
Milner, Y., Avigad, G. (1967) A copper reagent for the determination of hexuronic acids and certain ketohexoses. Carbohydr. Res. 4, 359
Moore, P.J., Swords, M.M., Lynch, M.A., Staehelin L.A. (1991) Spatial organization of the assembly pathways of glycoproteins and complex polysaccharides in the Golgi apparatus of plants. J. Cell Biol. 112, 589–602
Morrè, D.J. (1989) Sorting signals and trafficking of lysosomal and extracellular hydrolases of cell separation. In: Cell separation in plants (NATO ASI Series, vol. H35) pp. 81–99, Osborne, D.J., Jackson, M.B., eds. Springer-Verlag, Berlin, Heidelberg
Peterson, R.L., Peterson, C.A. (1986) Ontogeny and anatomy of lateral roots. In: New root formation in plants and cuttings, pp. 1–30, Jackson, M.B., ed. Martinus Nijoff publishers, Dordrecht, Boston, Lancaster
Pressey, R., Reger, B.J. (1989) Polygalacturonase in pollen from corn and other grasses. Plant Sci. 59, 57–62
Roberts, J.A., Taylor, J.E., Lasslett, Y.V., Tucker, G.A. (1989) Changes in gene expression during ethylene-induced leaf abscission. In: Cell separation in plants, (NATO ASI Series, vol. H35) pp. 61–68, Osborne, D.J., Jackson, M.B., eds. Springer-Verlag, Berlin, Heidelberg
Scott-Craig, J.S., Panaccione, D.G., Cervone, F., Walton, J.D. (1990) Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize. Plant Cell 2, 1191–1200
Taylor, J.E., Tucker, G.A., Lasslett, Y., Smith, C.J.S., Arnold, C.H., Watson, C.F., Schluch, W., Grierson, D., Roberts, J.A. (1990) Polygalacturonase expression during leaf abscission of normal and transgenic tomato plants. Planta 183, 133–138
Sutcliffe, J.F., Sexton, R. (1968) β-Glycerophosphatase and lateral root development. Nature 217, 1285
Van den Bosch, K.A., Bradley, D.J., Knox, J.P., Perotto, S., Butcher, G.W., Brewin, N.J. (1989) Common components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots. EMBO J. 8, 335–342
Van Tieghem, P., Douliot, H. (1888) Recherches comparatives sur l'origine des membres endogènes dans les plantes vasculaires. Ann. Sci. Nat. Bot. 8, 1–660
Vonhöne, H. (1880) Ueber das Hervorbrechen endogener Organe aus dem Mutterorgane. Flora 63, 268–274
Wilson, K.J., Nessler, C.L., Mahlberg, P.G. (1976) Pectinase in Asclepias latex and its possible role in laticifer growth and development. Am. J. Bot. 63, 1140
Wing, R.A., Yamaguchi, J., Larabell, S.K., Ursin, V.M., McCormick, S. (1990) Molecular and genetic characterization of two pollen expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia. Plant Mol. Biol. 14, 17–28
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The Authors are grateful to Dr. Dean Della Penna (Department of Vegetable Crops, University of California, Davis, Calif., USA) for generously providing the polyclonal antibody raised against the tomato polygalacturonase. This research was supported by National Research of Italy, Special project RAISA, Subproject N∘2, N∘360.
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Peretto, R., Favaron, F., Bettini, V. et al. Expression and localization of polygalacturonase during the outgrowth of lateral roots in Allium porrum L.. Planta 188, 164–172 (1992). https://doi.org/10.1007/BF00216810
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DOI: https://doi.org/10.1007/BF00216810