Summary
The localization of somatostatin-like immunore-activity (SOM-LI) was examined in human lumbar sympathetic ganglia using the peroxidase-antiperoxidase method. Few of the principal neurons showed immunolabelling for somatostatin and sparse networks of nerve terminals were unevenly associated with ganglion cells. Using light microscopy, the most intense SOM-LI was seen in the perinuclear zone of the neurons. Electron-microscopically, the staining was localized on the membranes of the Golgi apparatuses. In the nerve terminals, SOM-LI was seen inside the small vesicles (40–60 nm diameter). All neurons with SOM-LI were also found to be tyrosine-hydroxylase immunoreactive when excamined with a double-staining technique. These results provide evidence that somatostatin and noradrenaline co-exist in human sympathetic neurons.
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References
Brazeau P, Vale W, Burgus R, Ling N, Butcher M, Rivier C, Guillemin R (1973) Hypothalamic polypeptide that inhibits the secretion of immunoreactive growth hormone. Science 179:77–79
Burnweit C, Forssmann WG (1979) Somatostatinergic nerves in the cervical spinal cord of the monkey. Cell Tissue Res 200:83–90
Cooper PE, Fernström HM, Rorstad OP, Leeman SE, Martin HB (1981) The regional distribution of somatostatin, substance P and neurotensin in human brain. Brain Res 218:219–232
Dalsgard CJ, Hökfelt T, Johansson O, Elde R (1981) Somatostatin immunoreactive cell bodies in the dorsal horn and the parasympathetic intermediolateral nucleus of the rat spinal cord. Neurosci Lett 27:335–339
Davies P, Thompson A (1981) Postmortem stability of somatostatin-like immunoreactivity in mouse brain under conditions simulating handling of human autopsy material. Neurochem Res 6:787–791
Fried G (1982) Neuropeptide storage in vesicles. In: Klein RL, Lagercrantz H, Zimmerman H (eds) Neurotransmitter vesicles. Academic Press, London, pp 361–374
Fried G, Terenius L, Brodin E, Efendic S, Dockray G, Goldstein M, Hökfelt T (1986) Neuropeptide Y, enkephalin and noradrenaline coexist in sympathetic neurons innervating the bovine spleen. Cell Tissue Res 243:459–508
Hancock MB (1982) Leu-enkephalin, substance P and somatostatin immunohistochemistry combined with retrograde transport of horseradish peroxidase in sympathetic preganglionic neurons. J Auton Nerv Syst 6:263–272
Hökfelt T, Elde R, Johansson O, Luft R, Arimura A (1975) Immunohistochemical evidence of the presence of somatostatin, a powerful inhibitory peptide, in some primary sensory neurons. Neurosci Lett 1:231–235
Hökfelt T, Elfvin LG, Elde R, Schultzberg M, Goldstein M, Luft R (1977) Occurrence of somatostatin-like immunoreactivity in some peripheral sympathetic noradrenergic neurons. Proc Natl Acad Sci USA 74:3587–3591
Järvi R, Helen P, Pelto-Huikko M, Hervonen A (1986) Neuropeptide Y (NPY)-like immunoreactivity in rat sympathetic neurons and small granule containing cells. Neurosci Lett 67:223–227
Johansson O (1978) Localization of somatostatin-like immunoreactivity in the Golgi apparatus of central and peripheral neurons. Histochemistry 58:167–176
Lehy T, Peanzi G, Cristina ML (1984) Correlative immunocytochemical and electron microscopic studies: Identification of (entero)-glucagon, somatostatin and pancreatic polypeptide-like cells in the human colon. Histochemistry 71:67–80
Leranth C, Williams TH, Jew JY, Arimura A (1980) Immunoelectron microscopic identification of somatostatin in cells and axons of sympathetic ganglia in guinea pig. Cell Tissue Res 212:83–89
Lundberg JM, Hamberger B, Schultzberg M, Hökfelt T, Grandberg PO, Efendic S, Terenius L, Goldstein M, Luft R (1979) Enkephalin and somatostatin-like immunoreactivities in human adrenal medulla and pheochromocytoma. Proc Natl Acad Sci USA 76:4079–4083
Pickel VM, Joh TH, Reis DJ, Leeman SE, Miller RJ (1979) Electron microscopic localization of substance P and enkephalin in axon terminals related to dendrites of catecholaminergic neurons. Brain Res 160:387–400
Richardson KC (1966) Electron microscopic identification of autonomic nerve endings. Nature 210:756
Role LW, Leeman SE, Perlman RL (1981) Somatostatin and substance P inhibit catecholamine secretion from isolated cells of guinea-pig adrenal medulla. Neuroscience 6:1813–1821
Sjölund K, Sanden G, Håkandson R, Sundler F (1983) Endocrine cells in human intestine: An immunohistochemical study. Gastroenterology 85:1120–1130
Sternberger LA (1979) Immunohistochemistry, 2nd ed. Wiley, New York, Toronto
Sternberger LA, Joseph SA (1979) The unlabeled antibody method.Contrasting color staining of paired pituitary hormones without antibody removal. J Histochem Cytochem 27:1424–1429
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Järvi, R., Pelto-Huikko, M., Helen, P. et al. Somatostatin-like immunoreactivity in human sympathetic ganglia. Cell Tissue Res. 249, 1–5 (1987). https://doi.org/10.1007/BF00215411
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DOI: https://doi.org/10.1007/BF00215411