Summary
Sera from transgenic mice (TM) carrying human genes of α1-acid glycoprotein (orosomucoid or ORM) have been analyzed by isoelectrofocusing and subsequent immuno-blotting with antihuman ORM antibodies. With this technique it is possible to reveal selectively the human protein secreted in the TM sera. Orosomucoid bands present in TM sera have been compared with those of the most common human ORM phenotypes to correlate the products of specific genes to previously identified genetic variants. In this paper, we report the identification of the genes encoding for variants ORM1 F1 and ORM2 A, which are genes AGP-A and AGP-B/B′ respectively. The nucleotide sequences of these genes are known; therefore a direct correlation between variants and specific amino acid sequences can be established.
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References
Cox D, Francke U (1985) Direct assignment of ORM to human chromosome 9 and 2-HS glycoprotein to chromosome 3 using foetal liver/rat hepatoma hybrids. Hum Genet 70:109–115
Dente L, Pizza MG, Metspalu A, Cortese R (1987) Structure and expression of the genes coding for human α1-acid glycoprotein. EMBO J 6:2289–2296
Dente L, Ruther U, Tripodi M, Wagner E, Cortese R (1988) Expression of human α1-acid glycoprotein in cultured cells and in transgenic mice. Genes Dev 2:259–266
Eap CB, Baumann P (1988) Isoelectric focusing of α1-acid glycoprotein (orosomucoid) in immobilized pH-gradients with 8M urea: detection of its desialylated variants using an alkaline phosphatase-linked secondary antibody system. Electrophoresis 9:650–654
Eap CB, Baumann P (1989) The genetic polymorphism of human α1-acid glycoprotein. In: Baumann P, Eap CB, Muller WE, Tillement JP (eds) α1-Acid glycoprotein: genetics, biochemistry, physiological functions and pharmacology. Liss, New York, pp 111–125
Johnson AM, Schmid K, Alper CA, Bissett L (1969) Inheritance of human α1-acid glycoprotein (orosomucoid) variants. J Clin Invest 48:2293–2299
Nimberg R, Motoyama T, Schmid K (1971) The amino acid substitutions found in the genetic variants of α1-acid glycoprotein. J Biol Chem 18:5817–5821
Palmiter RD, Brinster RL (1986) Transgenic mice. Annu Rev Genet 20:465–499
Rocchi M, Roncuzzi L, Santamaria R, Archidiacono N, Dente L, Romeo G (1986) Mapping through somatic cell hybrids and cDNA probes of protein C to chromosome 2, factor X to chromosome 13, and α1-acid glycoprotein to chromosome 9. Hum Genet 74:30–33
Schmid K (1975) Alpha1-acid glycoprotien. In: Putnam FW (ed) The plasma proteins. Academic Press, New York San Francisco London, pp 184–228
Yuasa I, Umetsu K, Suenaga K, Robinet-Levy M (1986) Orosomucoid (ORM) typing by isoelectric focusing: evidence for two strucural loci ORM1 and ORM2. Hum Genet 74:160–161
Weidinger S, Müller T, Schwarzfischer F, Cleve H (1987) Three new orosomucoid (ORM) variants revealed by isoelectric focusing and print immunofixation. Hum Genet 77:286–288
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Tomei, L., Eap, C.B., Baumann, P. et al. Use of transgenic mice for the characterization of human alpha 1-acid glycoprotein (orosomucoid) variants. Hum Genet 84, 89–91 (1989). https://doi.org/10.1007/BF00210681
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DOI: https://doi.org/10.1007/BF00210681