Abstract
Scrapings from phlyctens and conjunctiva of 12 patients with phlyctenular keratoconjunctivitis were studied using OKT4-Leu3a, OKT8, B1, BA1, S-100 and HLA-DR monoclonal antibodies. T-lymphocytes were present in both conjunctival and phlyctenular scrapings. OKT4-Leu3a positive cells outnumbered the OKT8 positive cells in both conjunctival and phlyctenular scrapings. B1 and BA1 positive cells were absent from the conjunctival scrapings, but were present in the phlyctenular scrapings. S-100 positive cells were present in both conjunctival and phlyctenular scrapings. However, they were very few in the conjunctival scrapings. Most of the cells in both conjunctival and phlyctenular scrapings were HLA-DR positive. These findings support the hypothesis that cell mediated immunity is responsible for the pathogenesis of phlyctenular eye disease.
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References
Abramson CS, Kersey JH, Lè Bien TW: A monoclonal antibody (BA1), reactive cwith cells of human B-lymphocyte lineage. J Immunol 126: 83–88, 1981
Al-Hussaini MK, Saoudi MS: The aetiology of phlyctenular eye disease. Proceedings of the Fourth Afro-Asian Congress of Ophthalmology, pp. 425–431, 1968
Allansmith MR: Phlyctenular keratoconjunctivitis. In: Duane TD (ed) Clinical Ophthalmology. Philadelphia, Harper & Row, Publishers, Vol. 4, 1982, pp. 1–5
Bhan AK, Fujikawa LS, Foster CS: T-cell subsets and Langerhans cells in normal and diseased conjunctiva. Am J Ophthalmol 94: 205–212, 1982
Davis PL, Watson JI, Sapp GA: Experimental phlyctenular keratitis. Canad J Ophthalmol 5: 284–292, 1970
Dawson CR: Immunology of ocular chlamydial infections. Rev Int Trachome 61: 13–23, 1984
Duke-Elder S: Diseases of the outer eye. In: Duke-Elder S (ed) System of Ophthalmology. London, Henry Kimpton. Vol. 8, 1965, pp. 461–475
Forsum U, Klareskog L, Peterson PA: Distribution of la-antigen-like molecules on non-lymphoid tissues. Scand J Immunol 9: 343–349, 1979
Fransden E: Eye diseases following BCG vaccination. Acta Ophthalmol 57: 13–16, 1959
Graham RC, Lundholm U, Karnovsky MJ: Cytochemical demonstration of peroxidase activity with 3-amino-9-ethylcarbazole. J Histochem Cytochem 13: 150–152, 1965
Hassan AM, El-Gammal J: Clinical aspects and therapy of phlyctenular keratoconjunctivitis. Bull Ophthalmol Soc Egypt 61: 283–291, 1968
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA: Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med 153: 310–323, 1981
Mason DY, Naiem M, Abdulaziz Z, Nash JRG, Gatter KC, Stein H: Immunohistochemical labelling of cryostat sections with monoclonal antibody. In: Mc Michael AJ, Fabre JW (eds) Monoclonal antibodies in clinical medicine. London, Academic Press, 1982, pp. 632–635
Mondino BJ, Kowalski RP: Phlyctenules and catarrhal infiltrates in rabbits immunized with staphylococcus cell walls. Arch Ophthalmol 100: 1968–1971, 1982
Mondino BJ, Cruz TA, Kowalski RP: Immune responses in rabbits with phlyctenules and catarrhal infiltrates. Arch Ophthalmol 101: 1275–1277, 1983
Morris HHB, Gatter KC, Stein H, Mason DY: Langerhans cells in human cervical epithelium: an immunohistochemical study. Br J Obstet Gynaecol 90: 400–411, 1983
Nadler LM, Stashenko P, Ritz J, Hardy R, Pesando JM, Schlossman SF: A unique cell surface molecule identifying lymphoid malignancies of B-cell origin. J Clin Invest 67: 134–140, 1981
Poulter LW, Janossy G: The involvement of dendritic cells in chronic inflammatory disease. Scand J Immunol 21: 401–407, 1985
Reinherz EL, Kung PC, Goldstein G, Schlossman SF: Further characterization of the human inducer T-cell subset defined by a monoclonal antibody. J Immunol 123: 2894–2896, 1979
Reinherz EL, Kung PC, Goldstein G, Schlossman SF: A monoclonal antibody reactive with the human cytotoxic/ suppressor T-cell subset previously defined by a heteroantiserum termed TH2. J Immunol 124: 1301–1307, 1980
Smolin G, O'Connor GR: Ocular immunology. Philadelphia, Lea and Febiger, 1981, pp. 214–231
Takahashi K, Isobe Ohtsuki Y, Sonobe H, Tokedo I, Akagi T: Immunohistochemical localization and distribution of S-100 proteins in the human lympho-reticular system. Am J Pathol 116: 497–503, 1984
Van den Oord JJ, De Wolf-Peeters C, Vanstapel MJ, Desmet VJ: Improved immunohistochemical visualization of helper/inducer T-cells by the simultaneous application of two non crossblocking monoclonal antibodies. Stain Technology 60: 45–49, 1985
Van der Valk P, Van der Loo EM, Jansen J, Daha MR, Meyer CJLM: Analysis of lymphoid and dendritic cells in human lymph node, tonsil and spleen. A study using monoclonal and heterologous antibodies. Virchows Arch (cell pathol) 54: 169–185, 1984
Vantrappen L, Geboes K, Missotten L, Maudgal PC, Desmet V: Lymphocytes and Langerhans cells in normal human cornea. Invest Ophthalmol Vis Sci 26: 220–225, 1985
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Abu El Asrar, A.M., Geboes, K., Maudgal, P.C. et al. Immunocytological study of phlyctenular eye disease. Int Ophthalmol 10, 33–39 (1987). https://doi.org/10.1007/BF00202779
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DOI: https://doi.org/10.1007/BF00202779