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Anatomical observations on the nucellar apex of Wellwitschia mirabilis and the chemical composition of the micropylar drop

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Summary

In the young ovule of Welwitschia mirabilis the nucellar apex is dome shaped and starch begins to accumulate near the female gametophyte. With the degeneration of the cells of the nucellar apex, a pollen chamber is formed, which contains the micropylar fluid. Starch storage increases considerably in the upper part of the nucellus. Pollen drop emission is not a rhythmic process, and pollination does not produce the rapid withdrawal of droplets. The micropylar drop consists almost entirely of sugars, uronic acids and a very small amount of free amino acids and enzymes. The mechanism of micropylar drop secretion and its probable role in the process of pollination is discussed.

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References

  • Blumen Krantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acid. Anal Biochem 54:484–489

    CAS  PubMed  Google Scholar 

  • Bornman CH (1972) Welwitschia mirabilis: paradox of the Namib desert. Endeavour 31:95–99

    Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Carafa AM, Melchionna M, Pizzolongo P (1988) Considerazioni sulla biologia ed ecologia di Welwitschia mirabilis Hook, coltivata nell'Orto botanico di Portici. G Bot Ital [Suppl 1] 121:86

    Google Scholar 

  • Carafa AM, Napolitano G, D'Acunzo A (1989) fesWelwitschia mirabilis Hook.: una strana pianta del deserto della Namibia. Nat Montagna 36:17–20

    Google Scholar 

  • Cutter EG (1978) Plant anatomy part I: cells and tissues. Edward Arnold London

    Google Scholar 

  • Duhoux E, Pham Thi A (1980) Influence de quelques acides amines libres de l'ovule sur la croissance et le dévelopment cellulaire “in vitro” du tube pollinique chez Juniperus communis (Cupressacées). Physiol Plant 50:6–10

    Article  CAS  Google Scholar 

  • Feder N, O'Brien TP (1968) Plant microtechnique: some principles and new methods. AM J Bot 55:123–142

    Google Scholar 

  • Herrero M, Dickinson HG (1979) Pollen — pistil incompatibility in Petunia hybrida: changes in the pistil following compatible and incompatible intraspecific crosses. J Cell Sci 36:11–18

    Google Scholar 

  • Knox RB (1984): Pollen-pistil interactions. In: Linskens HF, Heslop-Harrison (eds) Encyclopedia of plant physiology. (New series, vol 17: cellular interactions.) Springer, Berlin Heidelberg New York, pp 508–606

    Google Scholar 

  • Martens P, Waterkeyn L (1973) Étude sur les Gnetals — XIII-Recherches sur Welwitschia mirabilis — V — Evolution ovulaire et embryogenése. Cell 70:165–257

    Google Scholar 

  • McWilliam JR (1958) The role of the micropyle in the pollination of Pinus. Bot Gaz 120:109–117

    Google Scholar 

  • Moussel B (1980) Gouttelette réceptrice du pollen et pollinisation chez l'Ephedra distachya L. Observations sur le vivant et en microscopies photonique et électronique. Rev Cytol Biol Veg 3:65–89

    Google Scholar 

  • Owens JN, Simpson SJ, Molder M (1981) Sexual reproduction of Pinus contorta. I. Pollen development, the pollination mechanism and early ovule development. Can J Bot 59:1828–1843

    Google Scholar 

  • Rougier M (1972) Étude cytochimique des squamules d'Elodea canadensis. Mise in évidence de leur sécrétion polysaccharidique et de leur activité phosphatasique acide. Protoplasma 74:113–131

    Google Scholar 

  • Schmidt JL, Graffard C, Lenoir J (1979) Contribution a l'étude des aptitudes biochimiques de levures isolées du fromage de camembert. I Essais préliminaires. Le Lait 583–584:142–163

    Google Scholar 

  • Seridi R, Chesnoy L (1986) Ultrastructure et cytochimie des cellules superficielles du nucelle de Thuya orientalis L. (=Biota orientalis (L.) Endl) au moment de l'emission de la goutte micropylaire. Bull Soc Bot Fr Lett 133:111–124

    Google Scholar 

  • Seridi R, Chesnoy L (1988a) Secretion and composition of the pollination drop in the Cephalotaxus drupacea (Gymnosperm, Cephalotaxeae). In: Cresti M, Gori P, Pacini E (eds) Sexual reproduction in higher plants. Springer, Berlin Heidelberg New York Tokyo, pp 345–350

    Google Scholar 

  • Seridi R, Chesnoy L (1988b) Cytologie du nucelle du Cephalotaxus drupacea Siev. et Zucc., lors de la sécrétion de la goutte de pollination. Ann Sci Univ Reims Champ Arden-Arers 23:64–66

    Google Scholar 

  • Singh H (1978) Embryology of gymnosperms. (Encyclopedia of plant anatomy — Band 10, Teil 2.) Borntraeger, Berlin Stuttgart

    Google Scholar 

  • Sprio RG (1966) Determination of neutral sugars. In: Neufeld EF, Ginsburg V (eds) Methods in Enzymology, vol. 8. Academic Press, New York, pp 4–5

    Google Scholar 

  • Ziegler H (1959) Über die Zusammensetzung des Bestäubungstropfen und den Mechanismus seiner Sekretion. Planta 52:587–599

    Google Scholar 

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This work was supported by a grant from MURST 40%

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Carafa, A.M., Carratu', G. & Pizzolongo, P. Anatomical observations on the nucellar apex of Wellwitschia mirabilis and the chemical composition of the micropylar drop. Sexual Plant Reprod 5, 275–279 (1992). https://doi.org/10.1007/BF00197378

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