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Lack of adhesion molecules in testicular diffuse centroblastic and immunoblastic B cell lymphomas as a contributory factor in malignant behaviour

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Abstract

Diffuse large B-cell lymphoma of the testis is a rare tumour, often with disseminated disease. According to the Kiel Classification, these lymphomas are of centroblastic or immunoblastic type, corresponding in the Working Formulation to malignant lymphoma, large cell non-cleaved and large cell immunoblastic, respectively. Adhesive cell-cell and cell-matrix interactions are generally assumed to play an important part in the metastatic process, and to find clues to the highly malignant biological behaviour of this tumour we examined expression of integrins and other adhesion molecules on the tumour cells and the presence of matrix proteins. Few adhesion molecules appeared to be expressed. CD44 was expressed in 10/12 lymphomas, CD49f/VLA-6 was positive in 5/12 cases, and CD49d/VLA-4, CD54 and CD62L were detectable in a small number (2–3) of lymphomas. All other adhesion molecules were lacking. This expression pattern is suggestive of a high metastatic potential: the tumour cells seem to be poorly attached to the extracellular matrix, to each other and to other cells (CD54-, CD11a-, CD58-). The adhesion molecules expressed, CD44, CD49f/VLA-6 and CD49d/VLA-4, have been reported to play a part in dissemination, mediating intravasation (CD49f/VLA-6) and extravasation (CD44, CD49d/VLA-4). This profile of adhesion molecules may explain, at least in part, the specific biological behaviour of these lymphomas with early and rapid dissemination.

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References

  1. Boyd AW, Dunn SM, Fecondo JV, Gulvenor JG, Dührsen U, Burns GF, Wawryk SO (1989) Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood 73:1896–1903

    Google Scholar 

  2. Brittinger G, Bartels H, Common H, et al (1984) Clinical and prognostic relevance of the Kiel classification of non-Hodgkin's lymphomas. Results of a prospective multicenter study by the Kiel Lymphoma Study Group. Hematol Oncol 2:269–306

    Google Scholar 

  3. Doll DC, Weiss RB (1993) Malignant lymphoma of the testis. Am J Med 81:515–524

    Google Scholar 

  4. Duncan PR, Checa F, Gowing NFC, McElwain TJ, Peckman MJ (1980) Extranodal non-Hodgkin's lymphoma presenting in the testicle. A clinical and pathologic study of 24 cases. Cancer 45:1578–1584

    Google Scholar 

  5. Ferry JA, Harris NL, Young RH, Coen J, Zietman A, Scully RE (1994) Malignant lymphoma of the testis, epididymis, and spermatic cord: a clinicopathologic study of 69 cases with immunophenotypic analysis. Am J Surg Pathol 18:376–390

    Google Scholar 

  6. Gallatin WM, Weissman IL, Butcher EC (1983) A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 304:30–34

    Google Scholar 

  7. Haynes BF, Telen MJ, Hale LP, Denning SM (1989) CD44 — a molecule involved in leukocyte adherence and T-cell activation. Immunol Today 10:423–428

    Google Scholar 

  8. Hemler ME, Elices MJ, Parker C, Takada Y (1990) Structure of the integrin VLA-4 and its cell-cell and cell-matrix adhesion functions. Immunol Rev 114:45–66

    Google Scholar 

  9. Horst E, Meijer CJ, Radaszkiewicz T, Van Dongen JJ, Pieters R, Figdor CG, Hooftman A, Pals ST (1990) Expression of a human homing receptor (CD44) in lymphoid malignancies and related stages of lymphoid development. Leukemia 4:383–389

    Google Scholar 

  10. Horst E, Meijer CJLM, Radaszkiewicz T, Ossekoppele GJ, Van Krieken JHJM, Pals ST (1990) Adhesion molecules in the prognosis of diffuse large-cell lymphoma: expression of a lymphocyte homing receptor (CD44), LFA-1 (CD11a/18), and ICAM-1 (CD54). Leukemia 4:595–599

    Google Scholar 

  11. Horst E, Radaszkiewicz T, Hooftman-den Otter A, Pieters R, Van Dongen JJM, Meijer CJLM, Pals ST (1991) Expression of the leucocyte integrin LFA-1 (CD11a/CD18) and its ligand ICAM-1 (CD54) in lymphoid malignancies is related to lineage derivation and stage of differentiation but not to tumour grade. Leukemia 5:848–853

    Google Scholar 

  12. Inghirami G, Wieczorek R, Zhu B-Y, Silber R, Dalla-Favera R, Knowles DM (1988) Differential expression of LFA-1 molecules in non-Hodgkin's lymphoma and lymphoid leukemia. Blood 72:1431

    Google Scholar 

  13. Iwamoto Y, Robey FA, Graf J, Sasaki M, Kleinman HK, Yamada Y, Martin GR (1987) YIGSR, a synthetic laminin pentapeptide, inhibits experimental metastasis formation. Science 238:1132–1134

    Google Scholar 

  14. Jalkanen S, Joensuu H, Klemi P (1990) Prognostic value of lymphocyte homing receptor and S-phase fraction in non-Hodgkin's lymphoma. Blood 75:1549–1556

    Google Scholar 

  15. Koopman G, Pals ST (1992) Cellular interactions in the germinal center: role of adhesion receptors and significance for the pathogenesis of AIDS and malignant lymphoma. Immunol Rev 126:21–45

    Google Scholar 

  16. Kroese FGM, Timens W, Nieuwenhuis P (1990) Germinal center reaction and B lymphocytes: morphology and function. Curr Top Pathol 84:103–148

    Google Scholar 

  17. Maio M, Pinto A, Carbone A, Zagonel V, Gloghini A, Marotta G, Cirillo D, Colombatti A, Ferrara F, Del Vecchio L, Ferrone S (1990) Differential expression of CD54/intercellular adhesion molecule-1 in myeloid leukemias and in lymphoproliferative disorders. Blood 76:783–790

    Google Scholar 

  18. McCarthy JB, Skubitz APN, Iida J, Mooradian DL, Wilke MS, Furcht LT (1991) Tumor cell adhesive mechanisms and their relationship to metastasis. Cancer Biol 2:155–167

    Google Scholar 

  19. Medeiros LJ, Weiss LM, Picker LJ, Clayberger C, Horning SJ, Krensky AM, Warnke RA (1989) Expression of LFA-1 in non-Hodgkin's lymphoma. Cancer 63:255–259

    Google Scholar 

  20. Möller P, Eichelmann A, Koretz K, Mechtersheimer G (1992) Adhesion molecules VLA-1 to VLA-6 define discrete stages of peripheral B lymphocyte development and characterize different types of B cell neoplasia. Leukemia 6:256–264

    Google Scholar 

  21. Möller P, Eichelmann A, Leithäuser F, Mechtersheimer G, Otto HF (1992) Venular endothelium binding molecules CD44 and LECAM-1 in normal and malignant B-cell populations. A comparative study. Virchows Arch [A] 421:305–313

    Google Scholar 

  22. Non-Hodgkin's Lymphoma Pathologic Classification Project (1982) National Cancer Institute sponsored study of classifications of non-Hodgkin's lymphomas. Summary and description of a Working Formulation for clinical usage. Cancer 49:2112–2135

    Google Scholar 

  23. Nozawa Y, Yamaguchi Y, Tominaga K, Hojo H, Abe M, Wakasa H (1992) Expression of leukocyte adhesion molecules (ICAM-1/LFA-1) related to clinical behavior in B cell lymphomas. Hematol Oncol 10:189–194

    Google Scholar 

  24. Paladugu RR, Bearman RM, Rappaport H (1980) Malignant lymphoma with primary manifestation in the gonad: a clinicopathologic study of 38 patients. Cancer 45:561–571

    Google Scholar 

  25. Pals ST, Meijer CJLM, Radaszkiewicz T (1991) Expression of the human peripheral lymph node homing receptor (LECAM-1) in nodal and gastrointestinal non-Hodgkin's lymphomas. Leukemia 5:628–631

    Google Scholar 

  26. Picker LJ, Medeiros LJ, Weiss LM, Warnke RA, Butcher EC (1988) Expression of lymphocyte homing receptor antigen in non-Hodgkin's lymphoma. Am J Pathol 130:496–504

    Google Scholar 

  27. Ruoslahti E (1991) Integrins. J Clin Invest 87:1–5

    Google Scholar 

  28. Ruoslahti E, Giancotti FG (1989) Integrins and tumor cell dissemination. Cancer Cells 1:119–126

    Google Scholar 

  29. Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein RL, Springer TA, Tedder TF, Todd RF (1994) CD antigens 1993. Immunol Today 15:98–99

    Google Scholar 

  30. Spertini O, Freedman AS, Belvin MP, Penta AC, Griffin JD, Tedder TF (1991) Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells. Leukemia 5:300–308

    Google Scholar 

  31. Stauder R, Greil R, Schulz TF, Thaler J, Gattringer C, Radaszkiewicz T, Dierich MP, Huber H (1989) Expression of leucocyte function-associated antigen-1 and 7F7-antigen, an adhesion molecule related to intercellular adhesion molecule-1 (ICAM-1) in non-Hodgkin lymphomas and leukaemias: possible influence on growth pattern and leukaemic behaviour. Clin Exp Immunol 77:234–238

    Google Scholar 

  32. Timens W (1995) Cell adhesive molecule expression and homing of hematologic malignancies. Crit Rev Oncol Hematol 19:111–129

    Google Scholar 

  33. Van Krieken JHJM, Medeiros LJ, Pals ST, Raffeld M, Kluin PM (1992) Diffuse aggressive B-cell lymphomas of the gastrointestinal tract: an immunophenotypic and gene rearrangement analysis of 22 cases. Am J Clin Pathol 97:170–178

    Google Scholar 

  34. Wawryk SO, Novotny JR, Wicks IP, Wilkinson D, Maher D, Salvaris E, Welch K, Fecondo J, Boyd AW (1989) The role of the LFA-1/ICAM-1 interaction in human leukocyte homing and adhesion. Immunol Rev 108:135–161

    Google Scholar 

  35. Wilkins BS, Williamson JMS, O'Brien CJ (1989) Morphological and immunohistological study of testicular lymphomas. Histopathology 15:147–156

    Google Scholar 

  36. Wright DH (1989) Updated Kiel classification for lymphomas. J Pathol (Lond) 157:283–284

    Google Scholar 

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  1. Department of Pathology, University Hospital, University of Groningen, P.O. Box 30.001, NL-9700 RB, Groningen, The Netherlands

    W. G. Horstmann & W. Timens

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  1. W. G. Horstmann
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  2. W. Timens
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Horstmann, W.G., Timens, W. Lack of adhesion molecules in testicular diffuse centroblastic and immunoblastic B cell lymphomas as a contributory factor in malignant behaviour. Vichows Archiv A Pathol Anat 429, 83–90 (1996). https://doi.org/10.1007/BF00192429

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  • DOI: https://doi.org/10.1007/BF00192429

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