Summary
The effect of the cyanoacrylate tissue adhesive Histoacryl on new bone formation was studied in rats. Experimental heterotopic new bone formation was induced by implanting pieces of demineralized allogeneic bone matrix (DABM) in the abdominal wall of 10 growing Sprague-Dawley rats. This produces cartilage formation within and around the implants after 10 days, followed by enchondral ossification and the formation of an ossicle with remodelling bone and bone marrow after three weeks. Prior to implantation, the DABM-implants were treated with increasing amounts of the tissue adhesive n-Butyl-2-cyanoacrylate-monomer (Histoacryl). New bone formation was quantified at three weeks by assay of the ash content of the implants as a measure of net bone formation, by 45calcium uptake prior to sacrifice, and by histology. Treatment of DABM with the cyanoacrylate caused an intense inflammatory process with a foreign body reaction, and abolished bone induction and new bone formation. Tissue adhesives should be used with caution in fracture surgery since they inhibit new bone formation, cause a foreign body reaction, and may impede fracture healing.
Résumé
L'effet d'un tissu adhésif de cyanoacrylate (Histoacryl) sur la néoformation osseuse a été étudié chez le rat. Une néoformation osseuse hétérotopique a été induite par implantation de fragments de matrice osseuse allogène déminéralisée (MOAD) dans la paroi abdominale de 10 rats Sprague-Dowley en période de croissance. Cette implantation détermine en dix jours l'apparition de cartilage à l'intérieur et à la périphérie des implants, suivie d'une ossification enchondrale et de la formation d'os et de moelle osseuse à la fin de la troisième semaine. Avant d'être mis en place, les implants de MOAD ont été préparés par des quantités croissantes de n-butyl-2-cyanoacrylate monomère (Histoacryl). La formation d'os nouveau a été mesurée, à la troisième semaine, par l'analyse de la cendre des implants, par l'activité, avant sacrifice, du calcium 45 et par l'histologie. La préparation des implants de MOAD par le cyanoacrylate entraîne une réaction inflammatoire intense et arrête l'induction et la formation d'os nouveau. On peut en conclure que les tissue adhésifs doivent être utilisés avec précaution en chirurgie traumatologique car ils inhibent la néoformation osseuse, déterminent une réaction à corps étranger et peuvent retarder la consolidation des fractures.
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Ekelund, A., Nilsson, O.S. Tissue adhesives inhibit experimental new bone formation. International Orthopaedics 15, 331–334 (1991). https://doi.org/10.1007/BF00186872
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DOI: https://doi.org/10.1007/BF00186872