Abstract
Bovine cumulus-oocyte complexes with or without attached pieces of the mural stratum granulosum were matured with or without FSH in vitro for 24 h. Some complexes were ooplasmectomized (removal of ooplasm) at the onset of culture or after 9 h of culture, and in others the complete oocyte, including the zona pellucida, was removed. At the end of culture, the degree and morphology of corona and cumulus expansion were determined by a subjective scoring system, transmission electron microscopy followed by computerized image analysis, or scanning electron microscopy. In the absence of FSH no expansion was seen. In the presence of FSH, hyaluronidase-sensitive cumulus expansion was observed in intact complexes and complexes ooplasmectomized after 9 h of culture. In complexes ooplasmectomized at the start of culture, hyaluronidase-insensitive expansion of the corona compartment was seen as well. A similar reaction was found in free corona and cumulus cell masses cultured in the presence or absence of oocytes. Mural granulosa in intact and ooplasmectomized complexes expanded only slightly under the influence of FSH and exhibited no hyaluronidase sensitivity. Ooplasmectomy at the start of culture increased the frequency of ruffled cell membranes and decreased the occurrence of intercellular bridges as compared with unmanipulated complexes cultured under corresponding conditions. It is concluded that FSH-induced cumulus expansion and hyaluronidase-sensitive extracellular mucus production of bovine cumulus investment in vitro are not dependent on the oocyte, except for minor changes in the surface morphology of the cumulus cells.
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Buccione R, Vanderhyden BC, Caron PJ, Eppig JJ (1990) FSH-induced expansion of the mouse cumulus oophorus in vitro is dependent upon a specific factor(s) secreted by the oocyte. Dev Biol 138:16–25
Dekel N, Kraicer PF (1978) Induction in vitro of mucification of rat cumulus oophorus by gonadotrophin and adenosine 3′,5′-monophosphate. Endocrinology 102:1797–1802
Dekel N, Phillips DM (1979) Maturation of the rat cumulus oophorus. A scanning electron microscopic study. Biol Reprod 21:9–18
Eppig JJ (1979a) FSH stimulates hyaluronic acid synthesis by oocyte-cumulus cell complexes from mouse preovulatory follicles. Nature 281:483–484
Eppig JJ (1979b) Gonadotropin stimulation of the expansion of cumuli oophori isolated from mice: general conditions for expansion in vitro. J Exp Zool 208:345–353
Fléchon J-E, Motlík J, Hunter RHF, Flèchon B, Pivko J, Fulka J (1986) Cumulus oophorus mucification during resumption of meiosis in the pig. A scanning electron-microscope study. Reprod Nutr Dev 26:989–999
Fukui Y, Terawaki Y, Ono H (1982) Effects of gonadotropins on in vitro maturation of bovine follicular oocytes. Jpn J Fertil Steril 27:179–187
Gilula NB, Ebstein ML, Beers WH (1978) Cell-to-cell communication and ovulation: a study of the cumulus-oocyte complex. J Cell Biol 78:179–187
Gore-Langton RE, Daniel SAJ (1990) Follicle-stimulating hormone and estradiol regulate antrum-like reorganisation of granulosa cells in rat preantral follicle cultures. Biol Reprod 43:65–72
Hillensjö T, Channing CP (1980) Gonadotropin stimulation of steroidogenesis and cellular dispersion in cultured porcine cumuli oorphori. Gamete Res 3:233–240
Hyttel P (1987) Bovine cumulus-oocyte disconnection in vitro. Anat Embryol 176:41–44
Laurincík J, Picha J, Píchova D, Oberfranc M (1991a) Timing of laparoscopic aspiration of preovulatory oocytes in heifers. Theriogenology 35:415–423
Laurincík J, Picha J, Píchova D, Pivko J (1991b) Effect of LH-RH on meiotic maturation of preovulatory oocytes in superovulated heifers. Anim Reprod Sci 25:189–197
Laurincík J, Kroslák P, Hyttel P, Pivko J, Sirotkin AV (1992a) Bovine cumulus expansion and corona-oocyte dissconnection during culture in vitro. Reprod Nutr Dev 32:151–161
Laurincík J, Pivko J, Kroslák P (1992b) Cumulus oophorus expansion of bovine oocytes cultured in vitro: a SEM and TEM study. Reprod Dom Anim 27:217–228
Motlik J, Fulka J, Fléchon J-E (1986) Changes in intercellular coupling between pig oocytes and cumulus cells during maturation in vivo and in vitro. J Reprod Fertil 76:31–37
Murdoch WJ (1988) Disruption of cellular associations within the granulosal compartment of periovulatory ovine follicles: relationship to maturation of the oocyte and regulation by prostaglandins. Cell Tissue Res 252:459–462
Procházka R, Nagyová E, Rimkevicová Z, Nagai T, Kikuchi K, Motlik J (1991) Lack of effect of oocytectomy on expansion of the porcine cumulus. J Reprod Fertil 93:569–576
Salustri A, Yanagishita M, Hascall VC (1990) Mouse oocytes regulate hyaluronic acid synthesis and mucification by FSH-stimulated cumulus cells. Dev Biol 138:26–32
Süss U, Wüthrich K, Stranzinger G (1988a) Chromosome configurations and time sequence of the first meiotic division in bovine oocytes matured in vitro. Biol Reprod 38:871–880
Süss U, Kassner J, Müller M, Stranzinger G (1988b) Ultrastructure of the intercellular coupling of cumulus cells before and after in vitro maturation of bovine oocytes. Theriogenology 29:314
Süss U, Kassner J, Wütrich K, Stranzinger G (1990) Cumulus expansion, in vitro fertilization and embryonic development after in vivo maturation of bovine oocytes in the presence of folliclestimulating or luteinizing hormone. Reprod Dom Anim 25:3–13
Thibault C (1972) Final stages of mammalian meiotic maturation. In: Biggers JD, Shuetz AW (eds) Oogenesis. University Park Press, Baltimore, pp 397–411
Vanderhyden BC, Caron PhJ, Buccione R, Eppig JJ (1990) Development pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation. Dev Biol 140:307–317
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Laurincik, J., Hyttel, P. & Veselá, J. Ooplasmectomy has no influence on FSH-induced expansion of bovine cumulus investment in vitro. Anat Embryol 190, 65–72 (1994). https://doi.org/10.1007/BF00185847
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DOI: https://doi.org/10.1007/BF00185847