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Production of 2,3-butanediol in a membrane bioreactor with cell recycle

  • Biochemical Engineering
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Summary

The production of 2,3-butanediol by Enterobacter aerogenes DSM 30053 was studied in a cell recycle system with a microfiltration module. Emphasis was put on the influence of oxygen supply, cell residence time, dilution rate, and pH. Under optimal conditions a productivity as high as 14.6 g butanediol + acetoin/l per hour was achieved with a product concentration of 54 g/l and a product yield of 88%. This productivity is three times higher than that of an ordinary continuous culture. The achievable final product concentration of a cell recycle system was limited by the accumulation of the inhibiting by-product acetic acid, which increased very rapidly at low dilution rate. To maximize product concentration a fed-batch fermentation was carried out with stepwise pH adaption at high cell density. A final product concentration of 110 g/l was obtained with a productivity of 5.4 g/l per hour and a yield of 97%.

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References

  • Emerson RR, Flickinger MC, Tsao GT (1982) Kinetics of dehydration of aqueous 2,3-butanediol to methylethyl ketone. Ind Eng Chem Prod Res Dev 21:473–477

    Google Scholar 

  • Fond O, Jansen NB, Tsao GT (1985) A model of acetic acid and 2,3-butanediol inhibition of the growth and metabolism of Klebsiella oxytoca. Biotechnol Lett 7:727–732

    Google Scholar 

  • Hongo M, Nomura Y, Iwahara M (1986) Novel method of lactic acid production by electrodialysis fermentation. Appl Environ Microbiol 52:314–319

    Google Scholar 

  • Ledingham GA, Neish AC (1954) Fermentative production of 2,3-butanediol. In: Underkofler LA (ed) Industrial fermentations, vol 2. Chemical Publications, New York, pp 27–93

    Google Scholar 

  • Lee CW, Chang HN (1987) Kinetics of ethanol fermentation in membrane cell recycle fermentors. Biotechnol Bioeng 29:1105–1112

    Google Scholar 

  • Magee RJ, Kosaric N (1987) The microbial production of 2,3-butanediol. Adv Appl Microbiol 32:89–161

    CAS  Google Scholar 

  • Nomura Y, Iwahara M, Hongo M (1988) Acetic acid production by electrodialysis fermentation method with a computerized control system. Appl Environ Microbiol 54:137–142

    Google Scholar 

  • Ohleyer E, Blanch HW, Wilke CR (1985) Continuous production of lactic acid in a cell recycle reactor. Appl Biochem Biotechnol 11:317–332

    Google Scholar 

  • Park YS, Ohtake H, Fukaya M, Okumura H, Kawamura Y, Toda K (1989) Acetic acid production using a fermentor equipped with hollow fiber filter module. Biotechnol Bioeng 33:918–923

    CAS  Google Scholar 

  • Qureshi N, Cheryan M (1989) Production of 2,3-butanediol in a membrane recycle bioreactor. Process Biochem. 1989:172–175

  • Ramachandran KB, Goma G (1988) 2,3-Butanediol production from glucose by Klebsiella pneumoniae in a cell recycle system. J Biotechnol 9:39–46

    Google Scholar 

  • Sablayrolles JM, Goma G (1984) Butanediol production by Aerobacter aerogenes NRRL B199: effect of initial substrate concentration and aeration agitation. Biotechnol Bioeng 26:148–155

    CAS  Google Scholar 

  • Tegtmeier U (1989) Einsatz von Mikrofiltrationsmembranen zur Zellrückhaltung in einem kontinuierlichen Fermentationsprozess. Chem Ing Techn 61:570–571

    Google Scholar 

  • Tran-Dinh K, Chin CW, Hill F (1987) Zweistufiges kontinuierliches Verfahren zur Production von 2,3-Butandiol mit Klebsiella pneumoniae. Dechema-Jahrestagung der Biotechnologen, Frankfurt/Main

  • Watanabe T, Aoki T, Honda H, Taya M, Kobayashi T (1990) Production of ethanol in repeated-batch fermentation with membrane-type bioreactor. J Ferment Bioeng 69:33–38

    Google Scholar 

  • Yu EKC, Saddler JN (1983) Fed-batch approach to production of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations. Appl Environ Microbiol 44:777–784

    Google Scholar 

  • Zeng A-P, Biebl H, Deckwer W-D (1990a) 2,3-Butanediol production in continuous culture of Enterobacter aerogenes: role of oxygen supply. Appl Microbiol Biotechnol 33:264–268

    Google Scholar 

  • Zeng A-P, Biebl H, Deckwer W-D (1990b) Effect of pH and acetic acid on growth and 2,3-butanediol production of Enterobacter aerogenes in microaerobic culture. Appl Microbiol Biotechnol 33:485–489

    CAS  Google Scholar 

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Zeng, A.P., Biebl, H. & Deckwer, W.D. Production of 2,3-butanediol in a membrane bioreactor with cell recycle. Appl Microbiol Biotechnol 34, 463–468 (1991). https://doi.org/10.1007/BF00180571

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  • DOI: https://doi.org/10.1007/BF00180571

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