Summary
Quantitative J-correlation and triple-resonance ECOSY-type experiments are used to unambiguously establish the presence of four-bond sequential HN-Hα J-couplings in the protein staphylococcal nuclease. Substantially negative \({}^{\text{4}}{\text{J}}_{{\text{H}}^\alpha {\text{H}}^{\text{N}} } \) values, ranging from -0.8 to -2.3 Hz, are observed when the Ψ angle is near +120°, and the following ϕ angle near +60°. For other conformations, the four-bond HN-Hα J-couplings fall between -0.5 and +0.5 Hz.
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Vuister, G.W., Bax, A. Measurement of four-bond HN-Hα J-couplings in staphylococcal nuclease. J Biomol NMR 4, 193–200 (1994). https://doi.org/10.1007/BF00175247
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DOI: https://doi.org/10.1007/BF00175247