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Characteristics of 0/129-sensitive motile Aeromonas strains isolated from freshwater on starch-ampicillin agar

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Abstract

Motile Aeromonas hydrophila strains were recovered from several freshwater sources by spread-plating water samples on starch-ampicillin agar, originally described as a medium for recovering Aeromonas hydrophila quantitatively from foods. Starch-ampicillin agar was compared with membrane Aeromonas medium and Rimler-Shotts medium for selectivity for, and recovery of, Aeromonas strains from freshwater. Thirty-four Aeromonas strains thus isolated were identified to species level by their phenotypic characteristics, and the Mol% G+C of representative strains was determined. Although resistant to 10 μg of the vibriostatic agent 0/129, all these strains showed sensitivity to 150 μg 0/129, which brings into question the use of this agent for distinguishing aeromonads from vibrios. The ability of these strains to produce extracellular virulence factors was generally similar to that reported for environmental strains isolated by other methods from various geographical locations within and beyond Australia. Ten of the 20 A. sobria strains, but none of the A. hydrophila or A. caviae strains, produced enterotoxin as shown by the suckling mouse test. Haemolysin was produced by 9/10 of the enterotoxigenic A. sobria strains and 2/9 A. hydrophila strains. Hemagglutinating activity was detected in 5/20 A. sobria and 7/9 A. hydrophila strains, and was inhibited by fucose and mannose, but not by galactose. The characteristics of these strains were comparable with those of Aeromonas strains isolated from other freshwater environments apart from their sensitivity to 0/129.

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References

  1. Arcos ML, de Vincente A, Morinigo MA, Romero P, Borrego JJ (1988) Evaluation of several selective media for recovery of Aeromonas hydrophila from polluted waters. Appl Environ Microbiol 54:2786–2792

    Google Scholar 

  2. Atkinson HM, Trust TJ (1980) Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infect Immun 27:938–946

    Google Scholar 

  3. Barer MR, Millership SE, Tabaqchali S (1986) Relationships of toxin production to species in the genus Aeromonas. J Med Microbiol 22:303–309

    Google Scholar 

  4. Baumann P, Fumiss AL, Lee JV (1984) Genus 1. Vibrio Pacini 1854, 411AL. In: Kreig NR, Holt JG (eds). Bergey's manual of systematic bacteriology, vol. 1. The Williams & Wilkins Co, Baltimore, pp 518–539

    Google Scholar 

  5. Bryant TN, Lee JV, West PA, Colwell RR (1986) A probability matrix for the identification of species of Vibrio and related genera. J Appl Bacteriol 61:469–480

    Google Scholar 

  6. Buchanan RL, Palumbo SA (1985) Aeromonas hydrophila and Aeromonas sobria as potential food poisoning species: A review. J Food Safety 7:15–29

    Google Scholar 

  7. Burke V, Cooper M, Robinson J, Gracey M, Lesmana M, Echeverria P, Janda JM (1984) Haemagglutination patterns of Aeromonas spp. in relation to biotype and source. J Clin Microbiol 19:39–43

    Google Scholar 

  8. Burke V, Robinson J, Atkinson HM, Gracey M (1982) Biochemical characteristics of enterotoxigenic Aeromonas spp. J Clin Microbiol 15:48–52

    Google Scholar 

  9. Burke V, Robinson J, Berry RJ, Gracey M (1981) Detection of enterotoxins of Aeromonas hydrophila by a suckling-mouse test. J Med Microbiol 14:401–408

    Google Scholar 

  10. Cahill MM (1990) Virulence factors in motile Aeromonas species. J Appl Bacteriol 69:1–16

    Google Scholar 

  11. Daily OP, Joseph SW, Coolbaugh JC, Walker RI, Merrell BR, Rollins DM, Seidler RJ, Colwell RR, Lissner CR (1981) Association of Aeromonas sobria with human infection. J Clin Microbiol 13:769–777

    Google Scholar 

  12. Gracey M, Burke V, Robinson JR (1982) Aeromonas-associated gastroenteritis. Lancet ii: 1304–1306

    Google Scholar 

  13. Havelaar AH, During M, Versteegh JFM (1987) Ampicillin-dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration. J Appl Bacteriol 62:279–287

    Google Scholar 

  14. Hazen TC, Fliermans CB, Hirsch RP, Esch GW (1978) Prevalence and distribution of Aeromonas hydrophila in the United States. Appl Environ Microbiol 36:731–738

    Google Scholar 

  15. Kaper JR, Seidler RJ, Lockman H, Colwell RR (1979) Medium for the presumptive identification of Aeromonas hydrophila and Enterobacteriaceae. Appl. Environ Microbiol 36:1023–1026

    Google Scholar 

  16. Kirov SM, Rees B, Wellock RC, Goldsmid JM, Van Galen AD (1986) Virulence characteristics of Aeromonas spp. in relation to source and biotype. J Clin Microbiol 24:827–834

    Google Scholar 

  17. Lee JV, Hendrie MS, Shewan JM (1979) Identification of Aeromonas, Vibrio and related organisms. In: Identification methods for microbiologists, 2nd edition, Society for Applied Bacteriology Technical Series No. 14. Academic Press, London, pp 151–166

    Google Scholar 

  18. Lee JV, Shread P, Furniss AL, Bryant TN (1981) Taxonomy and description of Vibrio fluvialis sp. nov. (Synonym Group F Vibrios, Group EF 6). J Appl Bacteriol 50:73–94

    Google Scholar 

  19. Ljungh A, Wretlind B, Mollby R (1981) Separation and characterization of enterotoxin and two hemolysins from Aeromonas hydrophila. Acta Path Microbiol Scand Sect. B. 89:387–397

    Google Scholar 

  20. Mandel M, Marmur J (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology XII Part B:195–206

    Google Scholar 

  21. Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    CAS  PubMed  Google Scholar 

  22. Olivier G, Lallier R, Lariviere S (1981) A toxigenic profile of Aeromonas hydrophila and Aeromonas sobria isolated from fish. Can J Microbiol 27:330–333

    Google Scholar 

  23. Palumbo SA, Maxino F, Williams AC, Buchanan RL, Thayer DW (1985) Starch-ampicillin agar for the quantitative detection of Aeromonas hydrophila. Appl Environ Microbiol 50:1027–1030

    Google Scholar 

  24. Popoff M, Lallier R (1984) Biochemical and serological characteristics of Aeromonas. Methods in Microbiol 16:127–145

    Google Scholar 

  25. Popoff M, Veron M (1976) A taxonomic study of the Aeromonas hydrophila Aeromonas punctata group. J Gen Microbiol 94:11–22

    Google Scholar 

  26. Rippey SR, Cabelli VJ (1979) Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh waters. Appl Environ Microbiol 38:108–113

    Google Scholar 

  27. Rippey SR, Cabelli VJ (1985) Growth characteristics of Aeromonas hydrophila in limnetic waters of varying trophic state. Arch Hydrobiol 104:311–319

    Google Scholar 

  28. Santos Y, Toranzo AE, Dopazo CP, Nieto TP, Barja JL (1987) Relationships among virulence for fish, enterotoxigenicity, and phenotypic characteristics of motile Aeromonas. Aquaculture 67:29–39

    Google Scholar 

  29. Shotts EB Jr, Rimler R (1973) Medium for the isolation of Aeromonas hydrophila. App Microbiol 26:550–553

    Google Scholar 

  30. Sly LI, Blackall LL, Kraat PC, Tian-Shen T, Sangkhobol V (1986) The use of second derivative plots for the determination of Mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156

    Google Scholar 

  31. Thune RL, Graham TE, Riddle LM, Amborski RL (1982) Extracellular proteases from Aeromonas hydrophila. Partial purification and effects on age-0 channel catfish. Trans Am Fish Soc 3(6):749–754

    Google Scholar 

  32. Toranzo AE, Santos Y, Nieto TP, Barja JL (1986) Evaluation of different assay systems for identification of environmental Aeromonas strains. Appl Environ Microbiol 51:652–656

    Google Scholar 

  33. Turnbull PCB, Lee JV, Miliotis MD, van de Walle S, Koornhoff HJ, Jeffery LT, Bryant TN (1984) Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol 19:175–180

    Google Scholar 

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  1. Department of Microbiology, The University of Queensland, Q.4072, Australia

    Marian M. Cahill & I. C. MacRae

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  1. Marian M. Cahill
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  2. I. C. MacRae
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Cahill, M.M., MacRae, I.C. Characteristics of 0/129-sensitive motile Aeromonas strains isolated from freshwater on starch-ampicillin agar. Microb Ecol 24, 215–226 (1992). https://doi.org/10.1007/BF00174456

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  • DOI: https://doi.org/10.1007/BF00174456

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