Abstract
Perfluorodecalin, a perfluorocarbon (PFC), was used in this investigation as a dissolved oxygen carrier in the media of Streptomyces coelicolor cultures. The effects of different concentrations of PFC, PFC emulsified with pluronic F-68 and pluronic alone were investigated in the shake-flask cultures using both defined and complex media. In the defined medium with PFC alone, the maximum biomass and actinorhodin concentrations and the volumetric substrate consumption rates increased with increasing PFC concentration. They decreased dramatically, however, when the PFC concentration exceeded 50% (v/v). Emulsifying the PFC with pluronic F-68 resulted in a significant increase in antibiotic concentration while growth was unaffected. The inclusion of more than 4 g/l pluronic alone in the fermentation medium inhibited the growth. In the complex medium with 40% (v/v) PFC, although the final antibiotic concentration was unaffected, the onset of actinorhodin accumulation was 2 days earlier than that in the control. It was demonstrated that PFC and emulsified PFC did not have any deleterious effects on S. coelicolor cultures.
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Adlercreutz P, Mattiasson B (1982) Oxygen supply to immobilized cells. 3. Oxygen supply by haemoglobin or emulsions of perfluorochemicals. Eur J Appl Microbiol Biotechnol 16:165–170
Chandler D, Davey MR, Lowe KC, Mulligan BJ (1987) Effects of emulsified perfluorochemicals on growth and ultrastructure of microbial cells in culture. Biotechnol Let 9(3):195–200
Chibata I, Yamada S, Wada M, Izuo N, Yamaguchi T (1974) Cultivation of aerobic microorganisms. US Patent 3,850,753
Cho MH, Wang SS (1988) Enhancement of oxygen transfer in hybridoma cell culture by using a perfluorocarbon as an oxygen carrier. Biotechnol Let 10:855–860
Damiano D, Wang SS (1985) Novel use of a perfluorocarbon for supplying oxygen to aerobic submerged cultures. Biotechnol Let 7(2):81–86
Doull JL, Vining LC (1990) Nutritional control of actinorhodin production by Streptomyces coelicolor A3(2): suppressive effects of nitrogen and phosphate. Appl Microbiol Biotechnol 32:449–454
King AT, Mulligan BJ, Lowe KC (1989) Perfluorochemical and cell culture. Biotechnol 7:1037–1042
Mattiasson B, Adlercreutz P (1984) Use of perflurochemicals for oxygen supply to immobilized cells. Ann NY Acad Sci 413:545–547
Ozergin K, Mavituna F (1992) Factors affecting antibiotic production dynamics in Streptomycetes. The 1992 IChemE Research Event, IChemE Publications, Rugby, UK, pp 13–15
Ozergin-Ulgen K, Mavituna F (1993) Actinorhodin production by Streptomyces coelicolor A3(2): kinetic parameters related to growth, substrate uptake and production. Appl Microbiol Biotechnol 40:457–462
Ozergin-Ulgen K, Mavituna F (1994) Comparison of the activity of immobilised and freely suspended Streptomyces coelicolor A3(2). Appl Microbiol Biotechnol 41:197–202
Riess JG, Le Blanc M (1982) Solubility and transport phenomena in perfluorochemicals relevant to blood substitution and other biomedical applications. Pure Appl Chem 54:2383–2406
Rollins MJ, Jensen SE, Westlake WS (1988) Effect of aeration on antibiotic production by Streptomyces clavuligerus. J Ind Microbiol 3:357–464
Rudd BA, Hopwood DA (1979) Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2). J Gen Microbiol 114:35–43
Steel MR, Maxon WD (1966) Dissolved oxygen measurements in pilot- and production-scale novobiocin fermentations. Biotechnol Bioeng 8:97–108
Tremper KK, Anderson ST (1985) Perfluorochemical emulsion oxygen transport fluids: a clinical review. Annu Rev Med 36:309–313
Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ (1983) Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
Wright LF, Hopwood DA (1976) Identification of the antibiotic determined SCP1 plasmid of Streptomyces coelicolor A3(2). J Gen Microbiol 95:96–106
Zhang S, Handa-Corrigan A, Spier RE (1992) Oxygen transfer properties of bubbles in animal cell culture media. Biotechnol Bioeng 40:252–259
Zhou W, Rieger KH, Dors M, Schügerl K (1992) Influence of dissolved oxygen concentration on the biosynthesis of cephalosporin C. Enzyme Microb Technol 14:848–854
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Elibol, M., Mavituna, F. Effect of perfluorodecalin as an oxygen carrier on actinorhodin production by Streptomyces coelicolor A3(2). Appl Microbiol Biotechnol 43, 206–210 (1995). https://doi.org/10.1007/BF00172813
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DOI: https://doi.org/10.1007/BF00172813