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Large-scale production of anthocyanin by Aralia cordata cell suspension cultures

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Abstract

The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradation, was scaled up from flasks to a 10-1 glass jar fermentor, a 95-1 stainless steel jar fermentor, and finally a 500-l pilot-scale jar fermentor. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0–17.2% (w/w) of the dried cells. In one of the operations of the 500-l jar fermentor, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells).

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Correspondence to: Y. Kobayashi

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Kobayashi, Y., Akita, M., Sakamoto, K. et al. Large-scale production of anthocyanin by Aralia cordata cell suspension cultures. Appl Microbiol Biotechnol 40, 215–218 (1993). https://doi.org/10.1007/BF00170369

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  • DOI: https://doi.org/10.1007/BF00170369

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