Summary
Attempts were made to develop the vinegar fermentation process using a fixed-film rotating reactor. Based on the capability of tolerating high temperatures and of forming very thin films, Acetobacter sp. 249-1 was used. Cotton towel cloth was employed as the support. For cell fixation, coconut water was found to be a better substrate than synthetic medium. Prevention of contamination was achieved by addition of 0.8% ethanol and 1% acetic acid to the medium. As revealed by scanning electron microscopy, cells were densely fixed to the fibrils of the support material. Batch fermentation using a fixed film rotating disc reactor resulted in an acetic acid yield (Yp/s) of 0.95. The system generated acid at a production rate of 0.23 g/l per hour. The fixed cells gave this yield over 8 months of sequential batch operation.
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References
Adams MR (1985) Vinegar. In: Wood JB (ed) Microbiology of fermented foods, vol 1. Elsevier, London, pp 1–49
Allgeier RJ, Hilderbrandt FM (1960) Newer developments in vinegar manufacture. Adv Appl Microbiol 11:163–182
Anderson JG, Blain JA (1980) Novel developments in microbial film reactors. In: Smith JE, Berry DR, Kristiansen B (eds) Fungal biotechnology. Academic Press, London, pp 125–152
Antonie RL (1975) Fixed biological surface wastewater treatment. CRC Press, Cleveland
Boongorsrang A, Yoshimich M, Kenichi S (1982) Nitrogen removal throughout nitrification, followed by denitrification of a synthetic wastewater with a rotating disc contactor. J Ferment Technol 60:359–364
Borghi M, Converti DA, Parisi S, Ferraiolo G (1985) Continuous alcohol fermentation in an immobilized cell rotating disc reactor. Biotechnol Bioeng 27:761–768
Child R (1964) Coconuts. Longmans, London
Conner HA, Allgeier RJ (1976) Vinegar:its history and development. Adv Appl Microbiol 20:81–133
Deley J, Gillis M, Swings J (1984) Family Acetobacteriaceae. In: Krieg NR, Holt JG (eds) Bergey's manual of systematic bacteriology, vol. 1. William and Wilkins, Baltimore, pp 267–274
Ghommidh C, Navarro JM, Durand G (1982) Acetic acid production by immobilized Acetobacter cells:Oxygen transfer. Biotechnol Bioeng 24:605–617
Kenedy H (1980) Australian Patent Application A 6274/80
Lotong N (1983) Thai coconut vinegar. In: Steinkraus KH (ed) Handbook of indigenous fermented foods, vol 9. Dekker, New York, pp 413–414
Nanba A, Kimura K, Nagai S (1985) Vinegar production by Acetobacter ransens cells fixed on hollow fiber module. J Ferment Technol 63:175–179
Norris JR, Ribbons DW (1970) Methods in microbiology. Academic Press, New York
Ohmori S, Uozumi T, Beppu T (1982) Loss of acetic acid resistance and ethanol oxidizing ability in an Acetobacter strain. Agric Biol Chem 46:381–389
Okuhara A (1985) Vinegar production with Acetobacter grown on fibrous support. J Ferment Technol 63:57–60
Osuga J, Mori A, Kato J (1984) Acetic acid production by immobilized Acetobacter aceti cell entrapped in a k-carragenan gel. J Ferment Technol 62:139–149
Yasui Y, Ikoga W, Mori A, Date K, Adachi T (1973) US patent no. 3734746
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Lotong, N., Malapan, W., Boongorsrang, A. et al. Production of vinegar by Acetobacter cells fixed on a rotating disc reactor. Appl Microbiol Biotechnol 32, 27–31 (1989). https://doi.org/10.1007/BF00164818
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DOI: https://doi.org/10.1007/BF00164818