Abstract
An Arthrobacter nicotianae strain has been found to produce an inducible acyl coenzyme A (CoA) oxidase. Nine times more butyryl-CoA oxidase activity, compared to palmitoyl-CoA oxidase, was found in the cell extract. The addition of flavin adenine dinucleotide (FAD) caused an increase in acyl-CoA oxidase activity and thermal stability. The purified enzyme exhibited a relative molecular mass of 50 000 on sodium dodecyl sulphate-polyacrylamide gel electrophoresis and 100 000 under non-denaturing conditions. Acyl-CoA oxidase from Arthrobacter nicotianae is highly specific towards short-chain fatty acids. The fastest O2 uptake was observed with butyryl-CoA as substrate. The enzyme is inhibited by silver and mercury salts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bell RD, Bars RG, Gibson GG, Combi ChR (1991) Localization and differential induction of cytochrome P450IVA and acyl-CoA oxidase in rat liver. Biochem J 275:247–252
Butcher LA, Tomkins JK (1985) A comparison of silver staining methods for detecting proteins in ultrathin polyacrylamide gels on support film after isoelectric focusing. Anal Biochem 148:348–388
Cooper TG, Beevers H (1969) ß-Oxidation in glyoxysomes from castor bean endosperm. J Bio Chem 244:3514–3520
Dmochowska A, Dignard D, Maleszka R, Thomas DY (1990) Structure and transcriptional control of the Saccharomyces cerevisiae POX1 gene encoding acyl-coenzyme A oxidase. Gene 88:247–252
Greene RV, Gould JM (1984) Fatty acyl-coenzyme A oxidase activity and H2O2 production in Phanerochaete chrysosporium mycelia. Biochem Biophys Res Commun 118:437–443
Hill DE, Boulay R, Rogers D (1988) Complete nucleotide sequence of the peroxisomal acyl-CoA oxidase from the alkane-utilizing yeast Candida maltosa. Nucleic Acids Res 16:365–366
Hyrb D, Hogg JF (1979) Chain-length specificities of peroxisomal and mitochondrial ß-oxidation in rat liver. Biochem Biophys Res Commun 87:1200–1206
Jiang Z, Thorpe C (1983) Acyl-CoA oxidase from Candida tropicalis. Biochemistry 22:3752–3758
Murray WW, Rachubinski RA (1987) The primary structure of a peroxisomal fatty acyl-CoA oxidase from the yeast Candida tropicalis pK 233. Gene 51:119–128
O'Brien WJ, Frerman FE (1977) Evidence for a complex of three beta-oxidation enzymes in Escherichia coli: induction and localization. J Bacteriol 132:532–540
Osumi T, Hashimoto T, Ui N (1980) Purification and properties of acyl-CoA oxidase from rat liver. J Biochem 87:1735–1746
Perrela FW (1988) EZ-FIT: a practical curve-fitting microcomputer program for the analysis of enzyme kinetic data on IBM-PC compatible computers. Anal Biochem 174:437–447
Reubsaet FAG, Veerkamp JH, Bukkens SGF, Trijbels JMF, Monnens LAH (1988) Acryl-CoA oxidase activity and peroxisomal fatty acid oxidation in rat tissues. Biochim Biophys Acta 958:434–442
Sakayu S, Koji Y, Yoshiki T, Yamada H (1979) Acyl-CoA oxidase from Candida tropicalis. Biochem Biophys Res Commun 91:108–113
Schepers L, Van Veldhoven PP, Casteels M, Eyssen JH (1990) Presence of three acyl-CoA oxidases in rat liver peroxisomes. J Biol Chem 226:5242–5246
Schulz H (1991) Beta-oxidation of fatty acids. Biochim Biophys Acta 1081:109–120
Small GM, Lazarow PB (1987) Important of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis. J Cell Biol 105:247–250
Small GM, Szabo LJ, Lazarow PB (1988) Acyl-CoA oxidase contains two targeting sequences each of which can mediate protein important into peroxisomes. EMBO J 7:1167–1173
Thieringer R, Kunau W-H (1991a) The beta-oxidation system in catalase-free microbodies of the filamentous fungus Neurospora crassa. J Biol Chem 226:13110–13117
Thieringer R, Kunau W-H (1991b) Beta-oxidation system of the filamentous fungus Neurospora crassa. J Biol Chem 226:13118–13123
Van Roermund CWT, Brul S, Tager JM, Schutgens RBH, Wanders RJA (1991) Acyl-CoA oxidase, peroxisomal thiolase and dihydroxyacetone phosphate acyltransferase: aberrant subcellular localisation in zellweger syndrome. J Inherited Metab Disc 14:152–164
Wang R, Thorpe C (1991) The reductive half-reaction in acyl-CoA oxidase from Candida tropicalis: interaction with acyl-CoA analogues and an unusual thioesterase activity. Arch Biochem Biophys 286:504–510
Author information
Authors and Affiliations
Additional information
To Professor Dr. Helmut Simon for his 65th birthday
Correspondence to: H. Sztajer
Rights and permissions
About this article
Cite this article
Sztajer, H., Wagner, G. & Schmid, R.D. Bacterial short-chain acyl-CoA oxidase: production, purification and characterization. Appl Microbiol Biotechnol 39, 708–713 (1993). https://doi.org/10.1007/BF00164454
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00164454