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Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.

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Biotechnology Techniques

Summary

A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, β-actin,used as internal mRNA control.

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Del Giacco, L., Doneda, L., Grammatico, P. et al. Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.. Biotechnol Tech 9, 879–884 (1995). https://doi.org/10.1007/BF00158540

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  • DOI: https://doi.org/10.1007/BF00158540

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