Skip to main content
Log in

The preparation in vitro of chrysanthemum for transplantation to soil

4. The effects of eleven growth retardants on wilting

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Plantlets of Dendranthema grandiflora ‘Pennine Reel’ were grown from nodal sections on Sorbarods saturated with liquid medium containing 0–3 mg 1−1 of various growth retardants. After 4 weeks they were transferred to compost and maintained at a relative humidity of 42% at 27.5°C. Wilting was assessed over a period of 3 h. Plantlets treated with paclobutrazol, flurprimidol, triapenthenol, chlorphonium chloride, uniconazol and ancymidol showed dose-related reductions in wilting up to a concentration of 3 mg 1−1. Responses to tetcyclacis and mepiquat chloride were weaker, and no responses to chlormequat chloride, BTS 44584 or diaminozide could be detected. These observations are compatible with an hypothesis that resistance to wilting derives from inhibited synthesis of gibberellins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Asare-BoamhNK, HofstraG, FletcherRA & DumbroffEB (1986) Triadimefon protects bean plants from water stress through its effect on abscisic acid. Plant Cell Physiol. 27: 383–390

    Google Scholar 

  • DennisDT, UpperCD & WestCA (1965) An enzymic site of inhibition of gibberellin biosynthesis by AMO 1618 and other plant growth retardants. Physiol. Plant. 40: 948–952

    Google Scholar 

  • FletcherRA & HofstraG (1985) Triadimefon a plant multiprotectant. Plant Cell Physiol. 26: 775–780

    Google Scholar 

  • HenryMJ (1985) Plant growth regulating activity of sterol and gibberellin biosynthesis inhibitors. Bull. Plant Growth Reg. Soc. America 13 (2): 9–11

    Google Scholar 

  • KhanAA & FaustMA (1967) Growth retardants and α-amylase production. Physiol. Plant. 20: 673–681

    Google Scholar 

  • MurashigeT & SkoogF (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • RademacherW (1989) Gibberellins: metabolic pathways and inhibitors of biosynthesis. In: BogerP & SandmannG (Eds) Target Sites of Herbicide Action. (pp 127–145). CRC Press Inc., Boca Raton

    Google Scholar 

  • RobertsAV & SmithEF (1990) The preparation in vitro of chrysanthemum for transplantation to soil. 1. Protection of roots by cellulose plugs. Plant Cell Tiss. Org. Cult. 21: 129–132

    Google Scholar 

  • SantakumariM & FletcherRA (1987) Reversal of triazole-induced stomatal closure by gibberellic acid and cytokinins in Commelina benghalensis. Physiol. Plant. 71: 95–99

    Google Scholar 

  • SmithAR, ThomasTH & GarrodJF (1982) Specificity and mode of action of BTS 44584 and chlormequat chloride on wheat and soybeans III. The effect on endogenous gibberellin-like compounds. Ann. App. Biol. 101: 359–365

    Google Scholar 

  • SmithEF, RobertsAV & MottleyJ (1990a) The preparation in vitro of chrysanthemum for transplantation to soil. 2. Improved resistance to desiccation conferred by paclobutrazol. Plant Cell Tiss. Org. Cult. 21: 133–140

    Google Scholar 

  • SmithEF, RobertsAV & MottleyJ (1990b) The preparation in vitro of chrysanthemum for transplantation to soil 3. Improved resistance to desiccation conferred by reduced humidity. Plant Cell Tiss. Org. Cult. 21: 141–145

    Google Scholar 

  • SokalRR & RohlfFJ (1981) Biometry, Second Edition. W.H. Freeman & Co., New York

    Google Scholar 

  • SugavanamB (1984) Diastereoisomers and enantiomers of paclobutrazol: their preparation and biological activity. Pestic. Sci. 15: 296–302

    Google Scholar 

  • SwietlikD, MillerSS (1983) The effect of paclobutrazol on growth and response to water stress of apple seedlings. J. Amer. Soc. Hort. Sci. 108: 1076–1080

    Google Scholar 

  • WielandWF & WampleRL (1985) Root growth, water relations and mineral uptake of young ‘Delicious’ apple trees treated with soil-and stem-applied paclobutrazol. Scientia Hort. 26: 129–137

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Smith, E.F., Roberts, A.V., Mottley, J. et al. The preparation in vitro of chrysanthemum for transplantation to soil. Plant Cell Tiss Organ Cult 27, 309–313 (1991). https://doi.org/10.1007/BF00157596

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00157596

Key words

Navigation