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Extraction of extracellular polymer from anaerobic sludges

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Biotechnology Techniques

Summary

Two types of anaerobic sludge were analyzed for ECP (extracellular polymers) content under five extraction conditions. Results showed that EDTA was more effective than formaldehyde as an extractant. Increase of temperature and addition caustic also enhanced the extraction. The ratio between carbohydrate and protein fractions of ECP for both acetate- and benzoate-degrading sludge was 0.16–0.18. The former sludge had only 40–45% of ECP as in the latter sludge.

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References

  • APHA (1985). Standard Methods for the Examination of water and Wastewater, American Public Health Association, Washington D.C.

    Google Scholar 

  • Busch, P.L. and Stumm, W. (1968). Environ. Sci. Tech., 2, 49–53, (1968).

    Google Scholar 

  • Dubois, M., Giles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Anal. Chem. 28, 350–356.

    Google Scholar 

  • Eriksson, L and Hardin, A. M. (1984). Wat. Sci. Technol., 16, 55–68.

    Google Scholar 

  • Eriksson, L and Alm, B. (1991). Wat. Sci. Technol., 24, 21–28.

    Google Scholar 

  • Fang, H.H.P., Chui, H.K. and Li, Y.Y. (1995). Wat. Sci. Technol. 32(8), 165–172 (1995).

    Google Scholar 

  • Forster, C.F. and Clarke, A.R. (1983). Wat. Pollut. Control, 82, 430–433.

    Google Scholar 

  • Jia, X.S., Furumai, H. and Kusuda, T. (1991). J. Japan Sew. Works Assoc. Res., 28, 83–93.

    Google Scholar 

  • Lettinga, G., van Velsen, A.F.M., Hobma, S.M., de Zeeuw, W. and Klapwijk, A. (1980). Biotech. Bioengrg. 22, 699–734.

    Google Scholar 

  • Li, Y.Y., Fang, H.H.P., Chui, H.K. and Chen, T. (1995). J. Envrion. Engrg., 121(10), 748–751.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). J. Biol. Chem. 193, 265–275.

    Google Scholar 

  • Morgan, J.W., Forster, C.F. and Evison, L. (1990). Wat. Res., 24(6), 743–750.

    Google Scholar 

  • Nishikawa, S. and Kuriyama, M. (1968). Wat. Res., 2, 811–812.

    Google Scholar 

  • Pavoni, J.L., Tenney, M.W. and Echelberger, W.F. (1971). J. Wat. Pollut. Control Fed., 44, 414–431.

    Google Scholar 

  • Ross, W.R. (1984). Water SA, 10, 197–203.

    Google Scholar 

  • Ryssov-Nielson, H. (1975). Vatten, 31, 33–39, (1975).

    Google Scholar 

  • Sutherland, I.W. and Wilkinson, J.F. (1971). Methods in Microbiology, Vol. 5B, Chapter 5, Academic press, London and New York.

    Google Scholar 

  • Sutherland, I.W. (1985). A. Rev. Microbiol., 39, 243–270.

    Google Scholar 

  • Tenney, M.W. and Stumm, W.J. (1968). J. Water. Pollut. Control Fed., 39, 1370–1388.

    Google Scholar 

  • Tzeuka, Y. (1973). J. Wat. Pollut. Control Fed., 45, 531–536.

    Google Scholar 

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Fang, H.H.P., Jia, X.S. Extraction of extracellular polymer from anaerobic sludges. Biotechnol Tech 10, 803–808 (1996). https://doi.org/10.1007/BF00154662

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  • DOI: https://doi.org/10.1007/BF00154662

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