Summary
A simple procedure for screening Clostridium acetobutylicum for biotransformations to produce alcohols was developed by supplementing rich medium with glucose and butyric acid to obtain butanol-producing cultures. No special apparatus was required to maintain anaerobic conditions. The validity of the method was established by demonstrating that the cultures could reduce alicyclic and aromatic aldehydes and ketones to the corresponding alcohols.
Similar content being viewed by others
References
Bahl, H., Andersch, W., Braun, K. and Gottschalk, G. (1982). Eur. J. Appl. Microbiol. Biotechnol., 14, 17–20
Davies, H.G., Green, R.H., Kelly, D.R. and Roberts, S.M. (1989). Biotransformations in preparative organic chemistry, pp. 99–156, London: Academic Press.
Durre, P., Kuhn, A., Gottwald, M. and Gottschalk, G. (1987). Appl. Microbiol. Biotechnol. 26, 268–272
Gottschal J.C. and Morris, J.G. (1981). FEMS Microbiol. Lett. 12, 385–389
Gottwald, M., Hippe, H. and Gottschalk, G. (1984). Appl. Environ. Microbiol. 48, 573–576
Holt, R.A., Stephens, G.M. and Morris, J.G. (1984). Appl. Environ. Microbiol. 48, 1166–1170
Marvel, C.S. and Broderick, A.E. (1925). J. Am. Chem. Soc. 47, 3045–3051
Prescott S.G. and Dunn, C.G. (1959). Industrial microbiology, 3rd ed. New York: McGraw hill Book Co.
Rogers P. (1986). Adv. Appl. Microbiol. 31, 1–60
Simon H., Bader, J., Gunther, H., Neumann, S. and Thanos, J. (1985). Angew. Chem. Int. Ed. Engl. 24, 539–555
Stephens, G.M., Holt, R.A., Gottschal, J.C. and Morris, J.G. (1985). J. Appl. Bacteriol. 59, 597–605
Walter, K.A., Bennett, G.N. and Papoutsakis, E.T. (1992). J. Bacteriol. 174, 7149–7158
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Green, E.M., Kalil, M.S., Williams, P. et al. Screening for reduction of aldehydes and ketones by solventogenic cultures of the strict anaerobe, Clostridium acetobutylicum . Biotechnol Tech 8, 733–738 (1994). https://doi.org/10.1007/BF00151478
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00151478