Summary
Orthophosphate concentration of baker's yeast Saccharomyces cerevisiae was investigated during dynamic conditions. As an example for those dynamics in cell metabolism the transition from glucose limitation to glucose excess (Crabtree-effect) was choosen. As a result of the metabolic switch from complete to partial oxidative metabolism, the cytoplasmic phosphate concentration increased suddenly from 8.4 mM to a maximum of 17.5 mM and transiently decreased to a minimum of 7.0 mM.
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Theobald, U., Mohns, J. & Rizzi, M. Dynamics of orthophosphate in yeast cytoplasm. Biotechnol Lett 18, 461–466 (1996). https://doi.org/10.1007/BF00143471
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DOI: https://doi.org/10.1007/BF00143471