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Immobilization of pepsinogen on Sepharose

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Summary

Porcine pepsinogen was attached to CNBr-activated-Sepharose 4B and self-activated to form Sepharose-pepsin. The maximum loading of pepsin on Sepharose was about 43 mg of pepsin per g of freeze-dried product. Sepharose-pepsin was stable on exposure to protein substrates and had a specific activity similar to that of the free enzyme.

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Authors and Affiliations

  1. Department of Biochemistry, Memorial university of Newfoundland, A1B 3X9, St. John's, Newfoundland, Canada

    K. M. Shamsuzzaman & N. F. Haard

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  1. K. M. Shamsuzzaman
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  2. N. F. Haard
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Shamsuzzaman, K.M., Haard, N.F. Immobilization of pepsinogen on Sepharose. Biotechnol Lett 6, 351–356 (1984). https://doi.org/10.1007/BF00138004

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  • DOI: https://doi.org/10.1007/BF00138004

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