Summary
The effect of heat-shock on cell growth and the induction of recombinant protein synthesis by a temperature-sensitive (ts) Chinese hamster ovary (CHO) cell line was investigated. An optimal regime of successive 2 hour heat-shocks (42°C) over 72 hours was found to simultaneously arrest cell growth and induce the synthesis of recombinant protein. The enhanced induction achieved from growth arrested cells may find application in the production of cytotoxic proteins.
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References
Bebbington, C.R. and Hentschel, C.C.G. (1987). In: DNA cloning volume III, a practical approach, D.M. Glover, ed. pp. 163–188, Oxford: IRL Press.
Bendig, M.M., Stephens, P.E., Cockett, M.I., and Hentschel, C.C. (1987). DNA 6, 343–352.
Bovenberg, W.A., Dauwerse, J.G., Pospiech, H.M., Van Buul-Offers, S.C., Van den Brande, J.L., and Sussenbach, J.S. (1990). Mol. Cell. Endocrinol. 74, 45–59.
Chang, P.Y., Bjornstad, K.A., Goncz, K., and Blakely, E.A. (1990). Int. J. Hyperther. 6, 105–116.
Cockett, M.I., Bebbington, C.R., and Yarranton, G.T. (1990). Bio/technology 8, 662–667.
Cotter, T.G., Glynn, J., and Lennon, S. (1993). (Unpublished)
Docherty, A.J.P., Lyons, A., Smith, B.J., Wright, E.M., Stephens, P.E., Harris, T.J.R., Murphy, G., and Reynolds, J.J. (1985). Nature 318, 66–69.
Dreano, M., Brochot, J., Myers, A., Cheng-Meyer, C., Rungger, D., Voellmy, R., and Bromley, P. (1986). Gene 49, 1–8.
Jenkins, N. and Hovey, A. (1993). Biotechnol. Bioeng. 42, 1029–1036.
Li, G.C. and Werb, Z. (1982). Proc. Natl. Acad. Sci. USA 79, 3218–3222.
Mizuno, S., Ohkawara, A., and Suzuki, K. (1989). Int. J. Hyperther. 5, 105–113.
Pallavicini, M.G., Rosette, C., Reitsma, M., Deteresa, P.S., and Gray, J.W. (1990). J. Cell Physiol. 143, 372–380.
Roti Roti, J.L., Mackey, M.A., and Higashikubo, R. (1992). Cell Proliferation 25, 89–99.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular cloning, a laboratory manual (New York: Cold Spring Harbor Laboratory Press).
Voellmy, R., Ahmed, A., Schiller, P., Bromley, P., and Rungger, D. (1985). Proc. Natl. Acad. Sci. USA 82, 4949–4953.
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Hovey, A., Bebbington, C.R. & Jenkins, N. Control of growth and recombinant protein synthesis by heat-shock in a mutant mammalian cell line. Biotechnol Lett 16, 215–220 (1994). https://doi.org/10.1007/BF00134614
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DOI: https://doi.org/10.1007/BF00134614